人尿中γ-羟基丁酸（液体X）的快速比色筛查试验

Rapid colorimetric screening test for gamma-hydroxybutyric acid (liquid X) in human urine.

作者信息

Alston William C, Ng Karno

机构信息

Southwestern Academy, 2800 Monterey Road, San Marino, CA 91108, USA.

出版信息

Forensic Sci Int. 2002 Apr 18;126(2):114-7. doi: 10.1016/s0379-0738(02)00050-6.

DOI:10.1016/s0379-0738(02)00050-6

PMID:12084486

Abstract

A rapid colorimetric test for the detection of gamma-hydroxybutyric acid (GHB) is described. The ferric hydroxamate test for ester detection has been adapted to detect GHB in human urine samples from a healthy female and a healthy male subject. The assay can be performed within 5 min and with a GHB detection limit of 0.5 mg/ml when 0.3 ml of human urine is used and a GHB detection limit of 0.1 mg/ml when 1 ml of human urine is used. The colored complex indicating the presence of GHB is purple according to the assay conditions. Test results are free from the interference by alcohol, phenolic compounds and other biological chemicals under the assay conditions. In addition, the colorimetric test is free from the potential false-positive test result that could result from physiological concentrations of GHB.

摘要

本文描述了一种用于检测γ-羟基丁酸（GHB）的快速比色法。用于酯检测的异羟肟酸铁试验已被改编用于检测来自一名健康女性和一名健康男性受试者的人类尿液样本中的GHB。当使用0.3 ml人类尿液时，该测定可在5分钟内完成，GHB检测限为0.5 mg/ml；当使用1 ml人类尿液时，GHB检测限为0.1 mg/ml。根据测定条件，表明存在GHB的有色络合物为紫色。在测定条件下，测试结果不受酒精、酚类化合物和其他生物化学物质的干扰。此外，比色法没有因生理浓度的GHB而可能导致的潜在假阳性测试结果。