健康人和糖尿病患者尿液中γ-羟基丁酸（GHB）及其异构体的内源性浓度。

Urinary endogenous concentrations of GHB and its isomers in healthy humans and diabetics.

作者信息

Shima Noriaki, Miki Akihiro, Kamata Tooru, Katagi Munehiro, Tsuchihashi Hitoshi

机构信息

Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi, Chuo-ward, Osaka 541-0053, Japan.

出版信息

Forensic Sci Int. 2005 May 10;149(2-3):171-9. doi: 10.1016/j.forsciint.2004.05.017.

DOI:10.1016/j.forsciint.2004.05.017

PMID:15749359

Abstract

Urinary endogenous concentrations of gamma-hydroxybutyric acid (GHB), alpha-hydroxybutyric acid (AHB) and beta-hydroxybutyric acid (BHB) have been investigated for both healthy humans and diabetics by using a newly optimized GC-MS procedure. The endogenous concentrations in healthy volunteers' urine ranged 0.16-2.14 microg/ml for GHB, 0.10-2.68 microg/ml for AHB and 8.51-34.7 microg/ml for BHB. In diabetics, the concentrations ranged 0.17-3.03 microg/ml for GHB, 0.14-124 microg/ml for AHB and 4.94-4520 microg/ml for BHB. Although notably elevated BHB and AHB concentrations were observed for severely uncontrolled diabetics, their GHB concentrations ranged within or near the range seen in healthy humans. The results of this study confirm the previously suggested 10 microg/ml cutoff concentration of urinary GHB to distinguish exogenous GHB, even for uncontrolled diabetic patients suffering severe ketoacidosis.

摘要

通过使用一种新优化的气相色谱 - 质谱法（GC - MS），对健康人和糖尿病患者尿液中γ - 羟基丁酸（GHB）、α - 羟基丁酸（AHB）和β - 羟基丁酸（BHB）的内源性浓度进行了研究。健康志愿者尿液中的内源性浓度范围为：GHB为0.16 - 2.14微克/毫升，AHB为0.10 - 2.68微克/毫升，BHB为8.51 - 34.7微克/毫升。在糖尿病患者中，浓度范围为：GHB为0.17 - 3.０3微克/毫升，AHB为0.14 - 124微克/毫升，BHB为4.94 - 4520微克/毫升。虽然在严重未控制的糖尿病患者中观察到BHB和AHB浓度显著升高，但他们的GHB浓度在健康人所见范围内或接近该范围。本研究结果证实了先前建议的尿GHB 10微克/毫升的截断浓度，以区分外源性GHB，即使对于患有严重酮症酸中毒的未控制糖尿病患者也是如此。