人心血管组织中的神经肽Y Y1和神经肽Y Y2受体。
Neuropeptide Y Y1 and neuropeptide Y Y2 receptors in human cardiovascular tissues.
作者信息
Uddman Rolf, Möller Sebastian, Nilsson Torun, Nyström Susanne, Ekstrand Jonas, Edvinsson Lars
机构信息
Department of Otorhinolaryngology, University Hospital, Malmö, Sweden.
出版信息
Peptides. 2002 May;23(5):927-34. doi: 10.1016/s0196-9781(02)00003-7.
mRNA encoding the human NPY Y1 and NPY Y2 receptors were detected in cerebral, meningeal, and coronary arteries using reverse transcriptase-polymerase chain reaction (RT-PCR). In addition, the trigeminal and superior cervical ganglia were positive for both receptors. In some arteries and in SK-N-MC cells only mRNA encoding the NPY Y1 was detected. Besides the expected NPY Y1 PCR products, an additional 97 bp longer amplicon originating from an alternative splicing event was found in most tissues studied. Antibodies directed against the NPY Y1 receptor revealed immunostaining mainly in the smooth muscle layer of blood vessels whereas antibodies against the NPY Y2 receptor showed immunostaining in nerve cell bodies.
利用逆转录聚合酶链反应（RT-PCR）在脑动脉、脑膜动脉和冠状动脉中检测到了编码人神经肽Y Y1和神经肽Y Y2受体的信使核糖核酸（mRNA）。此外，三叉神经节和颈上神经节中的这两种受体均呈阳性。在一些动脉和SK-N-MC细胞中，仅检测到了编码神经肽Y Y1的mRNA。除了预期的神经肽Y Y1聚合酶链反应（PCR）产物外，在大多数研究组织中还发现了一个源自可变剪接事件的额外的、长97碱基对的扩增子。针对神经肽Y Y1受体的抗体显示免疫染色主要在血管平滑肌层，而针对神经肽Y Y2受体的抗体则在神经细胞体中显示免疫染色。
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