Claverol-Tinture Enric, Pine Jerome
Department of Physics, Mathematics and Astronomy, California Institute of Technology, 326 Kerchoff MC 156-29, 1200 East California Blvd., Pasadena, CA 91125, USA.
J Neurosci Methods. 2002 May 30;117(1):13-21. doi: 10.1016/s0165-0270(02)00043-2.
The detection of extracellular potentials by means of multi-electrode arrays (MEA) is a useful technique for multi-site long-term monitoring of cultured neuronal activity with single-cell resolution. To optimize the geometry of the MEA it is advantageous to localize the cellular compartments that constitute the generators of these signals. For this purpose, an in vitro technique for the detection of extracellular signals with subcellular resolution has been developed. It makes use of easy-to-manufacture large-tip pipettes, monitoring of electrode-cell gap resistance for precise electrode positioning and low-density (100 cells/mm(2)) dissociated hippocampal cultures. Negative monophasic extracellular spikes, typically 60 microV, were measured over putative axonal processes and monophasic, biphasic and triphasic signals were recorded over the soma. A compartmental simulation suggests that different somatic conductance densities of Na(+) (1-10 mS/cm(2)) and K(+) (5-10 mS/cm(2)) channels can produce characteristic somatic extracellular potentials, with a variety of shapes similar to those observed experimentally.
利用多电极阵列（MEA）检测细胞外电位是一种以单细胞分辨率对培养的神经元活动进行多位点长期监测的有用技术。为了优化MEA的几何形状，定位构成这些信号发生器的细胞区室是有利的。为此，已开发出一种用于以亚细胞分辨率检测细胞外信号的体外技术。它使用易于制造的大尖端移液管，监测电极-细胞间隙电阻以进行精确的电极定位，并使用低密度（100个细胞/mm²）解离的海马培养物。在假定的轴突过程中测量到负单相细胞外尖峰，通常为60微伏，在胞体上记录到单相、双相和三相信号。一个房室模拟表明，Na⁺（1-10 mS/cm²）和K⁺（5-10 mS/cm²）通道的不同胞体电导密度可以产生特征性的胞体细胞外电位，其形状多种多样，类似于实验中观察到的形状。
