Pichler Josefa, Gerstmayr Marianne, Szépfalusi Zsolt, Urbanek Radvan, Peterlik Meinrad, Willheim Martin
Department of Pediatrics, University of Vienna, A-1090 Vienna, Austria.
Pediatr Res. 2002 Jul;52(1):12-8. doi: 10.1203/00006450-200207000-00005.
Human naive CD4+ T helper (Th) and CD8+ cytotoxic (Tc) T cells, which only produce IL-2, may differentiate into Th1/Tc1- or Th2/Tc2-like lymphocytes, characterized by their cytokine production profile. 1 alpha,25-dihydroxyvitamin D3 (1 alpha, 25(OH)2D3) has been reported to inhibit Th1/Tc1-related, but increase Th2/Tc2-associated cytokines in T cells from adults. In industrialized countries, vitamin D supplementation for prevention of rickets is initiated within the first days of life and continued throughout the entire first year. Epidemiologic studies suggest an association of vitamin D exposure in newborns with the incidence of allergic diseases in later life. This study addresses the effects of 1 alpha, 25(OH)2D3 on Th1/Tc1 versus Th2/Tc2 differentiation in long term cell cultures of (naive) cord blood T lymphocytes. Our results show that in CD4+ as well as CD8+ cord blood cells, 1 alpha, 25(OH)2D3 inhibits not only IL-12-generated IFN-gamma production, but also suppresses IL-4 and IL-13 expression induced by IL-4. Thus, in cord blood 1 alpha, 25(OH)2D3 induces a T cell population without predominance of Th2 related cytokines.
人类初始CD4⁺辅助性T（Th）细胞和CD8⁺细胞毒性T（Tc）细胞仅产生白细胞介素-2（IL-2），它们可能分化为Th1/Tc1样或Th2/Tc2样淋巴细胞，其特征在于细胞因子产生谱。据报道，1α,25-二羟基维生素D3（1α,25(OH)2D3）可抑制成人T细胞中与Th1/Tc1相关的细胞因子，但增加与Th2/Tc2相关的细胞因子。在工业化国家，出生后几天内就开始补充维生素D以预防佝偻病，并在整个第一年持续补充。流行病学研究表明，新生儿维生素D暴露与后期生活中过敏性疾病的发病率有关。本研究探讨了1α,25(OH)2D3对（初始）脐血T淋巴细胞长期细胞培养中Th1/Tc1与Th2/Tc2分化的影响。我们的结果表明，在CD4⁺以及CD8⁺脐血细胞中，1α,25(OH)2D3不仅抑制IL-12诱导的γ干扰素（IFN-γ）产生，还抑制IL-4诱导的IL-4和IL-13表达。因此，在脐血中，1α,25(OH)2D3诱导出一个不占主导地位的Th2相关细胞因子的T细胞群体。
