Fisher Matthew C, Rannala Bruce, Chaturvedi Vishnu, Taylor John W
The Institute of Zoology, Regent's Park, London NW1 4RY, United Kingdom.
Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):9067-71. doi: 10.1073/pnas.132178099.
Molecular surveillance of pathogenic microbes works by genotyping isolates with DNA fingerprinting techniques and then using these genotypes to assign individuals to populations. Clonality is assumed in many fingerprinting studies, although this assumption has been shown to be false for many organisms. To accommodate recombining organisms into surveillance programs, methods using population allele frequencies in combination with individual multilocus genotypes are necessary. Here, we develop a statistical method appropriate for haploid recombining microbes that allows individuals to be assigned to populations. We illustrate the usefulness of this technique by inferring the source populations for Coccidioides isolates recovered from patients treated outside the endemic area of Coccidioides sp., the etiological agents of human coccidioidomycosis, but with a travel history including visits to one or more endemic areas.
对致病微生物进行分子监测的方法是，利用DNA指纹技术对分离株进行基因分型，然后使用这些基因型将个体归类到不同群体中。许多指纹分析研究都假定存在克隆性，尽管已证明这一假设对许多生物来说是错误的。为了将发生重组的生物纳入监测计划，需要采用结合群体等位基因频率和个体多位点基因型的方法。在此，我们开发了一种适用于单倍体重组微生物的统计方法，该方法能够将个体归类到不同群体中。我们通过推断从球孢子菌病流行区以外接受治疗的患者身上分离出的球孢子菌分离株的来源群体，来说明这项技术的实用性。球孢子菌是人类球孢子菌病的病原体，这些患者有前往一个或多个流行区的旅行史。
