Wilson I B
Institut für Chemie, Universität für Bodenkultur, Muthgasse 18, A-1190 Wien, Austria.
Glycoconj J. 2001 Jun;18(6):439-47. doi: 10.1023/a:1016030000527.
Recently, it has been found that plants, including tomato (Lycopersicon esculentum), express the Lewis-a epitope, Galbeta1,3(Fucalpha1,4)GlcNAc, on some N-glycans. By searching the EST database, it was possible to identify a tomato cDNA encoding a protein, designated FucTC, of 413 amino acids with homology to plant and mammalian alpha1,3/4-fucosyltransferases. The cDNA was expressed in Pichia pastoris and the recombinant enzyme was found to transfer fucose from GDP-Fuc (K(m) 16 microM) to lacto-N-tetraose (Galbeta1,3GlcNAcbeta1,3Galbeta1,4Glc; K(m) 80 microM) as well as to beta1,3- and beta1,4-galactosylated N-glycans. It is concluded that FucTC is responsible for the biosynthesis of Lewis-a on N-glycans in tomato.
最近发现，包括番茄（Lycopersicon esculentum）在内的植物在一些N - 聚糖上表达Lewis - a表位，即Galβ1,3（Fucα1,4）GlcNAc。通过搜索EST数据库，得以鉴定出一个番茄cDNA，其编码一种名为FucTC的蛋白质，该蛋白质有413个氨基酸，与植物和哺乳动物的α1,3/4 - 岩藻糖基转移酶具有同源性。该cDNA在毕赤酵母中表达，发现重组酶能将GDP - Fuc（Km为16μM）中的岩藻糖转移至乳糖 - N - 四糖（Galβ1,3GlcNAcβ1,3Galβ1,4Glc；Km为80μM）以及β1,3 - 和β1,4 - 半乳糖基化的N - 聚糖上。得出的结论是，FucTC负责番茄中N - 聚糖上Lewis - a的生物合成。
Zotero 插件