Department of Plant Biology, Cornell University Ithaca, NY, USA.
Front Plant Sci. 2012 Jun 6;3:117. doi: 10.3389/fpls.2012.00117. eCollection 2012.
N-Glycosylation is a common form of eukaryotic protein post-translational modification, and one that is particularly prevalent in plant cell wall proteins. Large scale and detailed characterization of N-glycoproteins therefore has considerable potential in better understanding the composition and functions of the cell wall proteome, as well as those proteins that reside in other compartments of the secretory pathway. While there have been numerous studies of mammalian and yeast N-glycoproteins, less is known about the population complexity, biosynthesis, structural variation, and trafficking of their plant counterparts. However, technical developments in the analysis of glycoproteins and the structures the glycans that they bear, as well as valuable comparative analyses with non-plant systems, are providing new insights into features that are common among eukaryotes and those that are specific to plants, some of which may reflect the unique nature of the plant cell wall. In this review we present an overview of the current knowledge of plant N-glycoprotein synthesis and trafficking, with particular reference to those that are cell wall localized.
N-糖基化是真核蛋白翻译后修饰的一种常见形式，尤其在植物细胞壁蛋白中更为普遍。因此，大规模和详细地描述 N-糖蛋白在更好地理解细胞壁蛋白质组的组成和功能，以及那些位于分泌途径其他部位的蛋白质方面具有相当大的潜力。虽然已经有许多关于哺乳动物和酵母 N-糖蛋白的研究，但对于它们的植物对应物的群体复杂性、生物合成、结构变异和运输了解较少。然而，糖蛋白分析技术的发展以及它们所承载的聚糖结构，以及与非植物系统的有价值的比较分析，正在为在真核生物中普遍存在的特征以及那些特定于植物的特征提供新的见解，其中一些可能反映了植物细胞壁的独特性质。在这篇综述中，我们介绍了植物 N-糖蛋白合成和运输的最新知识概述，特别提到了那些定位于细胞壁的糖蛋白。
