Soh Tomoki, Inoue Yoshie, Xi Yong-Mei, Kato Yukio, Hattori Masa-Aki
Faculty of Agriculture, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Asian J Androl. 2002 Jun;4(2):83-6.
The production of interspecific germline chimeras between chicken and quail were attempted employing the dissociated cells derived from the blastodermal central disk (stage X) and the germinal crescent region of embryo (stage 7-8).
The central disk (CD) of the area pellucida in chicken blastoderm (stage X) and the germinal crescent region (GCR) of embryo (stage 7-8) were dispersed and injected into the subgerminal cavity of quail blastoderm (stage X). Injected eggs were incubated for 7 days or to hatching. The donor chicken DNA was detected by the polymerase chain reaction.
In day-7 embryos, chicken DNA was detected in 5 gonads and 9 brains from 53 survived embryos received chicken CD cells, and 1 gonads and 6 brains from 27 survived embryos received chicken GCR. Chicken DNA was also detected from the semen of one adult male hatched from eggs received chicken GCR cells.
CD and GCR cells as the donors showed the possibility to produce the interspecific germline chimera, but further studies are needed to make necessary improvement.
尝试利用来自胚盘中央盘（X期）和胚胎生殖新月区（7-8期）的解离细胞，在鸡和鹌鹑之间产生种间生殖系嵌合体。
将鸡胚盘（X期）明区的中央盘（CD）和胚胎（7-8期）的生殖新月区（GCR）分散，并注入鹌鹑胚盘（X期）的胚下腔。将注射后的卵孵化7天或直至孵化。通过聚合酶链反应检测供体鸡的DNA。
在7日龄胚胎中，从接受鸡CD细胞的53只存活胚胎中的5个性腺和9个大脑中检测到鸡DNA，从接受鸡GCR的27只存活胚胎中的1个性腺和6个大脑中检测到鸡DNA。从接受鸡GCR细胞的卵孵化出的一只成年雄性的精液中也检测到鸡DNA。
作为供体的CD和GCR细胞显示出产生种间生殖系嵌合体的可能性，但需要进一步研究以进行必要的改进。
