Liu C H, Chang I K, Sasse J, Dumatol C J, Basker J V, Wernery U
Department of Cell Biology, Central Veterinary Research Laboratory, Dubai, United Arab Emirates.
Anim Reprod Sci. 2007 Oct;101(3-4):344-50. doi: 10.1016/j.anireprosci.2006.12.021. Epub 2007 Jan 8.
In present study, chicken primordial germ cells (PGCs) were transferred into quail embryos to investigate the development of these germ cells in quail ovary. Briefly, 2 microl of chicken embryonic blood (stage 14) or about 100 purified circulating PGCs were transferred into quail embryo. Contribution of chicken PGCs were detected in gonads of chimeric quail embryos (stage 28) by immunocytochemical staining of cell surface antigen SSEA-1, and by in situ hybridization (ISH) with female chicken specific DNA probe. As a result, 52.0+/-43.2 (n=18) and 42.7+/-27.3 (n=17) chicken PGCs were found in the gonads of chimeric quail embryo that was injected with chicken embryonic blood (stage 14) and about 100 purified circulating PGCs, respectively. Furthermore, the ovaries of 81.8% (9/11) 12 days post incubation (dpi) chimeric quail embryos were observed with a mean of 457.6+/-237.1 female chicken PGCs-derived oogonia scattered in ovarian cortex area. In 9 out of 12 newly hatched and one week old chimeric quail chicks, on average of 2883.0+/-1924.1 primary oocytes and 3 follicles derived from chicken PGCs were found, respectively. The present results suggest that chicken female PGCs are able to migrate, colonize, proliferate and differentiate into oogonia, primary oocytes in chimeric quail ovary.
在本研究中，将鸡原始生殖细胞（PGCs）移植到鹌鹑胚胎中，以研究这些生殖细胞在鹌鹑卵巢中的发育情况。简要来说，将2微升鸡胚胎血液（第14阶段）或约100个纯化的循环PGCs移植到鹌鹑胚胎中。通过对细胞表面抗原SSEA - 1进行免疫细胞化学染色以及用雌性鸡特异性DNA探针进行原位杂交（ISH），在嵌合鹌鹑胚胎（第28阶段）的性腺中检测鸡PGCs的贡献。结果发现，在分别注射了鸡胚胎血液（第14阶段）和约100个纯化循环PGCs的嵌合鹌鹑胚胎的性腺中，发现了52.0±43.2（n = 18）和42.7±27.3（n = 17）个鸡PGCs。此外，观察到81.8%（9/11）孵化后12天（dpi）的嵌合鹌鹑胚胎的卵巢中，平均有457.6±237.1个源自雌性鸡PGCs的卵原细胞散布在卵巢皮质区域。在12只新孵化和1周龄的嵌合鹌鹑雏鸡中的9只中，平均分别发现了2883.0±1924.1个初级卵母细胞和3个源自鸡PGCs的卵泡。目前的结果表明，鸡雌性PGCs能够在嵌合鹌鹑卵巢中迁移、定植、增殖并分化为卵原细胞、初级卵母细胞。
