Marques Adriana R, Martin Dale S, Philipp Mario T
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
J Clin Microbiol. 2002 Jul;40(7):2591-3. doi: 10.1128/JCM.40.7.2591-2593.2002.
The C6 enzyme-linked immunosorbent assay (ELISA), based on a peptide (C6) that reproduces the sequence of invariable region 6 of VlsE, the antigenic variation protein of Borrelia burgdorferi, has been shown to be a sensitive and specific test for the serologic diagnosis of Lyme disease. We now report that none of 29 uninfected individuals vaccinated with the recombinant OspA vaccine had an antibody response to the C6 peptide. The C6 peptide ELISA can be used to diagnose Lyme disease in patients who have received the OspA vaccine.
基于一种肽(C6)的C6酶联免疫吸附测定(ELISA),该肽复制了伯氏疏螺旋体抗原变异蛋白VlsE恒定区6的序列,已被证明是莱姆病血清学诊断的一种灵敏且特异的检测方法。我们现在报告,29名接种重组OspA疫苗的未感染个体中,没有一人对C6肽产生抗体反应。C6肽ELISA可用于诊断接种过OspA疫苗的患者的莱姆病。