[通过流式细胞术对智利慢性恰加斯病患者外周血淋巴细胞进行免疫表型分析]
[Immunophenotyping of peripheral blood lymphocytes from Chilean chronic chagasic patients by flow cytometry].
作者信息
Sánchez Gittith, Zulantay Inés, Gajardo Marta, Venegas Juan, Villanueva Roxana, Pérez Andrés, Liendo Federico, Apt Werner, Solari Aldo
机构信息
Programa Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile.
出版信息
Rev Med Chil. 2002 Apr;130(4):363-7.
BACKGROUND
Cellular immune mechanisms of the resistance to infection by T cruzi as well as the pathogenesis of Chagas disease are still controversial.
AIM
To quantify and analyse the peripheral blood immune cells from chagasic and non chagasic patients by flow cytometry.
PATIENTS AND METHODS
Peripheral blood samples were taken from 21 individuals seropositive for Chagas disease, under no specific treatment. Control samples from 21 healthy blood donors were also obtained. To quantify immune cells populations by flow cytometry, antibodies against CD3, CD4, CD8, CD16/56, CD45/14, CD19 and HLA-DR markers were used.
RESULTS
The percentage of CD8+ cells was low and the CD4+/CD8+ ratio was high in chagasic patients, compared to their non infected counterparts. No statistically significant differences in the number of CD4+, NK, B, CD4+HLADR+ and CD8+HLADR+ cells, were observed within the two groups.
CONCLUSIONS
These results show that Chilean chronic chagasic patients have lower percentage of CD8+ cells and higher CD4+/CD8+ ratio than non infected individuals.
背景
对克氏锥虫感染的抵抗力的细胞免疫机制以及恰加斯病的发病机制仍存在争议。
目的
通过流式细胞术对恰加斯病患者和非恰加斯病患者的外周血免疫细胞进行定量和分析。
患者与方法
采集21例未经特殊治疗的恰加斯病血清阳性个体的外周血样本。还获取了21名健康献血者的对照样本。为了通过流式细胞术对免疫细胞群体进行定量，使用了针对CD3、CD4、CD8、CD16/56、CD45/14、CD19和HLA-DR标志物的抗体。
结果
与未感染的对照组相比，恰加斯病患者中CD8+细胞的百分比低，CD4+/CD8+比值高。两组之间在CD4+、自然杀伤细胞（NK）、B细胞、CD4+HLADR+和CD8+HLADR+细胞数量上未观察到统计学上的显著差异。
结论
这些结果表明，智利慢性恰加斯病患者的CD8+细胞百分比低于未感染个体，CD4+/CD8+比值高于未感染个体。
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