Denny T N, Scolpino A, Garcia A, Polyak A, Weiss S N, Skurnick J H, Passannante M R, Colon J
Departments of Pediatrics and Pathology, New Jersey Medical School, Newark, USA.
Cytometry. 1995 Aug 1;20(4):349-55. doi: 10.1002/cyto.990200411.
The purpose of this study was to accurately determine the T-lymphocyte subsets found in semen from healthy volunteers, to evaluate the impact of repeated ejaculation on the frequency or type of immune cells present in semen, and to compare subset analysis in semen to that in the peripheral blood. To accomplish this, a flow cytometric method was developed to identify and count immunophenotypically distinct cells present in semen. Fresh semen samples and peripheral blood were collected over three consecutive days from nine healthy donors. Donors had normal ejaculate volume, sperm count, sperm motility, morphology, and leukocyte count. No significant intra-donor differences were seen in these parameters over time. No significant differences were observed in the percentage of CD3+ cells, CD4+ cells, CD8+ cells, and the CD4:CD8 ratio in semen on consecutive days. However, within the CD4+ subset, when naive and memory CD4+ cells were measured, some day to day variability was suggested. No significant differences in CD3+, CD4+, CD8+, CD4/CD8 ratio, or naive and memory subsets were seen in the peripheral blood between sampling days. When semen was compared to peripheral blood some differences in immune subset values were observed, with an increase in the percentage of memory CD4+ cells in semen being the most striking. This finding may be relevant to HIV transmission, since others have shown that this cell may be preferentially infected with HIV and is the primary reservoir for virus in infected individuals.
本研究的目的是准确测定健康志愿者精液中的T淋巴细胞亚群，评估重复射精对精液中免疫细胞频率或类型的影响，并比较精液与外周血中的亚群分析结果。为实现这一目的，开发了一种流式细胞术方法来识别和计数精液中免疫表型不同的细胞。连续三天从九名健康供体收集新鲜精液样本和外周血。供体的射精量、精子计数、精子活力、形态和白细胞计数均正常。随着时间的推移，这些参数在供体内未观察到显著差异。连续几天精液中CD3+细胞、CD4+细胞、CD8+细胞的百分比以及CD4:CD8比值均未观察到显著差异。然而，在CD4+亚群中，当测量初始和记忆性CD4+细胞时，提示存在一些每日变化。在采样日之间，外周血中CD3+、CD4+、CD8+、CD4/CD8比值或初始和记忆亚群均未观察到显著差异。当将精液与外周血进行比较时，观察到免疫亚群值存在一些差异，精液中记忆性CD4+细胞百分比的增加最为显著。这一发现可能与HIV传播有关，因为其他人已经表明，这种细胞可能优先感染HIV，并且是感染个体中病毒的主要储存库。
