Lie A K, Skarsvåg S, Haugen O A, Skjeldestad F E, Olsen A O, Skovlund E, Rønningen K S
Department of Pathology and Institute of Morphology, Faculty of Medicine, NTNU, Trondheim, Norway.
Int J Gynecol Pathol. 1999 Jul;18(3):206-10. doi: 10.1097/00004347-199907000-00003.
This study describes the distribution of DQB1genes in Norwegian women treated for high-grade cervical intraepithelial neoplasia (CIN). Formalin-fixed, paraffin-embedded tissue sections from 170 biopsy specimens with diagnoses of CIN II (n = 54) or CIN III (n = 116) were DQB1-typed using allele-specific polymerase chain reaction. The follow-up period for cases was 13 to 15 years. The control material comprised blood samples and endocervical brushes from 213 women without CIN. Both cases and controls had previously been human papillomavirus (HPV)-typed. The DQB10301 allele was overrepresented among cases compared with controls (odds ratio [OR] = 1.8). Presence of CIN was related to HPV infection, and HPV 16 positivity was significantly associated with the presence of DQB10301 (OR 1.8). The DQBI0301 allele was significantly more prevalent in CIN III than in CIN II cases. The lesions in two women recurred in the follow-up period, one of whom was carrying the DQB10301 allele. Women carrying the HLA-DQB1*0301 allele have an increased risk of developing CIN when infected by HPV 16, although there was not an increased frequency of recurrent disease among women carrying this allele.
本研究描述了接受高级别宫颈上皮内瘤变（CIN）治疗的挪威女性中DQB1基因的分布情况。对170例诊断为CIN II（n = 54）或CIN III（n = 116）的活检标本的福尔马林固定、石蜡包埋组织切片，采用等位基因特异性聚合酶链反应进行DQB1分型。病例的随访期为13至15年。对照材料包括213名无CIN女性的血液样本和宫颈内刷。病例和对照之前均已进行人乳头瘤病毒（HPV）分型。与对照相比，病例中DQB10301等位基因的比例过高（优势比[OR]=1.8）。CIN的存在与HPV感染有关，HPV 16阳性与DQB10301的存在显著相关（OR 1.8）。DQBI0301等位基因在CIN III中比在CIN II病例中更为普遍。两名女性的病变在随访期复发，其中一人携带DQB10301等位基因。携带HLA-DQB1*0301等位基因的女性在感染HPV 16时发生CIN的风险增加，尽管携带该等位基因的女性中复发病例的频率并未增加。
