印度女性中人类白细胞抗原II类等位基因与人类乳头瘤病毒介导的宫颈癌之间的关联。

Association between human leukocyte antigen class II alleles and human papillomavirus-mediated cervical cancer in Indian women.

作者信息

Kohaar Indu, Hussain Showket, Thakur Nisha, Tiwari Pratibha, Nasare Vilas, Batra Swaraj, Singh Veena, Bhambani Suresh, Das Bhudev C, Sarkar Debi P, Bharadwaj Mausumi

机构信息

Division of Molecular Genetics and Biochemistry, Institute of Cytology and Preventive Oncology (ICMR), I-7, Sector 39, Noida 201 301, India.

出版信息

Hum Immunol. 2009 Apr;70(4):222-9. doi: 10.1016/j.humimm.2009.01.003. Epub 2009 Jan 18.

DOI:10.1016/j.humimm.2009.01.003

PMID:19272325

Abstract

We investigated the association of human leukocyte antigen (HLA) II (DRB1 and DQB1) alleles with susceptibility to human papillomavirus (HPV)-associated cervical precancer and cancer cases in a hospital-based case-control study in a northern Indian population. A total of 202 subjects, including 100 patients comprising 31 cervical precancer (cervical intraepithelial neoplasia [CIN] 2/3) and 69 invasive cervical cancer cases, and 102 healthy controls participated in the study. Both patients and controls were screened for HPV infection using a polymerase chain reaction (PCR-based approach. Low-resolution PCR-sequence specific priming (PCR-SSP) was used to genotype HLA II (DRB1 and DQB1). Our results demonstrate that the DRB115 allele/DRB115-DQB106 haplotype may have a predisposition for HPV infection (p(c) < 0.05) or cervical cancer/precancer (p(c) < 0.05) development, whereas the DRB104 allele/DRB104-DQB103 haplotype might exhibit susceptibility to cervical precancerous lesions (p(c) < 0.05). The DRB113 allele/DRB113-DQB1*06 haplotype was strongly protective against risk to HPV infection (p(c) < 0.002) as well as cervical cancer (p(c) 0.01). Therefore, we have demonstrated that HLA DR-DQ polymorphisms are involved in genetic susceptibility to cervical cancer or HPV infection in a northern Indian population.

摘要

在一项针对印度北部人群的基于医院的病例对照研究中，我们调查了人类白细胞抗原（HLA）II类（DRB1和DQB1）等位基因与人类乳头瘤病毒（HPV）相关的宫颈上皮内瘤变和癌症易感性之间的关联。共有202名受试者参与了该研究，其中包括100例患者，其中31例为宫颈上皮内瘤变（CIN 2/3），69例为浸润性宫颈癌患者，以及102名健康对照。使用聚合酶链反应（基于PCR的方法）对患者和对照进行HPV感染筛查。采用低分辨率PCR序列特异性引物（PCR-SSP）对HLA II类（DRB1和DQB1）进行基因分型。我们的结果表明，DRB115等位基因/DRB115-DQB106单倍型可能易患HPV感染（p(c)<0.05）或宫颈癌/癌前病变（p(c)<0.05），而DRB104等位基因/DRB104-DQB103单倍型可能对宫颈上皮内瘤变易感（p(c)<0.05）。DRB113等位基因/DRB113-DQB1*06单倍型对HPV感染风险（p(c)<0.002）以及宫颈癌（p(c) 0.01）具有很强的保护作用。因此，我们证明了HLA DR-DQ多态性参与了印度北部人群对宫颈癌或HPV感染的遗传易感性。