Blanco A, Flores-Acuña F, Roldán-Villalobos R, Monterde J G
Department of Comparative Anatomy and Pathological Anatomy, Veterinary Faculty, University of Córdoba, 14071 Córdoba, Spain.
Vet J. 2002 May;163(3):292-8. doi: 10.1053/tvjl.2001.0693.
The morphological consequences of anabolic clenbuterol treatment on the testicular parenchyma were investigated in 30 pigs at morphological and ultrastructural levels. Clenbuterol was given with food (1 ppm). In the first group (n=10), treatment was maintained until slaughter (experimental period 3 months). In the second group (n=10), clenbuterol was withdrawn 2 weeks before slaughter (experimental period 2.5 months). A third group (n=10) of pigs not fed with clenbuterol served as controls. Animals were slaughtered at 9 months of age and samples of testicular parenchyma were collected for light and electron microscope studies. In the clenbuterol-treated groups, the interstitial cells showed a considerable increase in the organelles involved in testosterone production, with an increased development of the mitochondria, smooth endoplasmic reticulum, Golgi apparatus and lipid droplets compared to the control group. The seminal epithelium displayed many lipid vacuoles and evident signs of tubular involution, such as degenerating and multinucleate germ cells. Sertoli cells gave evidence of metabolic alterations such as large lipid deposits and cytolysosomes.
在30头猪身上，从形态学和超微结构水平研究了合成代谢类克伦特罗处理对睾丸实质的形态学影响。克伦特罗与食物一起投喂（1 ppm）。第一组（n = 10）持续处理至屠宰（实验期3个月）。第二组（n = 10）在屠宰前2周停止投喂克伦特罗（实验期2.5个月）。第三组（n = 10）未投喂克伦特罗的猪作为对照。动物在9月龄时屠宰，采集睾丸实质样本用于光镜和电镜研究。在克伦特罗处理组中，间质细胞中参与睾酮生成的细胞器显著增加，与对照组相比，线粒体、滑面内质网、高尔基体和脂滴的发育增加。生精上皮显示出许多脂质空泡以及明显的生精小管退化迹象，如生殖细胞退化和多核现象。支持细胞有代谢改变的迹象，如大量脂质沉积和溶酶体。
