Blanco Alfonso, Artacho-Pérula Emilio, Flores-Acuña Rafaela, Moyano Rosario, Monterde José G
Department of Comparative Anatomy and Pathological Anatomy, Veterinary Faculty, University of Córdoba, 14075 Córdoba, Spain.
Vet Immunol Immunopathol. 2003 Nov 15;96(1-2):111-5. doi: 10.1016/s0165-2427(03)00162-4.
Although the beta2 adrenergic agonists have been seen to have important effects on the mechanisms regulating the development and death of T-cells in the thymus, the side-effects on the immune system of anabolic treatments of these substances have hardly been considered. In order to evaluate the effects exerted by the beta2 adrenergic agonist clenbuterol on the thymocyte population, the thymus of eight pigs treated with anabolic doses of this substance was studied by morphometric methods, regarding apoptotic (terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL)-positive) and normal (TUNEL-negative) cells. The thymus of another eight pigs fed without clenbuterol served as a control. The clenbuterol treatment had a clear effect on the thymocyte size, decreasing their mean nuclear area. The T-cell apoptosis index was also affected by the clenbuterol, significantly increasing the apoptosis percentage in the treated group with respect to the control. In the light of our results, the clenbuterol induced thymocyte apoptosis throughout the thymus and caused morphometric changes in the thymocyte population, which was in line with the immunosuppressive role attributed to other beta2 adrenergic agonists.
尽管已发现β2肾上腺素能激动剂对胸腺中调节T细胞发育和死亡的机制具有重要影响，但这些物质的合成代谢治疗对免疫系统的副作用却几乎未被考虑。为了评估β2肾上腺素能激动剂克伦特罗对胸腺细胞群体的影响，采用形态计量学方法研究了八只用该物质合成代谢剂量处理的猪的胸腺，观察凋亡细胞（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）阳性）和正常细胞（TUNEL阴性）。另外八只未喂食克伦特罗的猪的胸腺作为对照。克伦特罗处理对胸腺细胞大小有明显影响，使其平均核面积减小。T细胞凋亡指数也受克伦特罗影响，与对照组相比，处理组的凋亡百分比显著增加。根据我们的结果，克伦特罗诱导整个胸腺的胸腺细胞凋亡，并导致胸腺细胞群体的形态计量学变化，这与归因于其他β2肾上腺素能激动剂的免疫抑制作用一致。
