Bredel Markus, Piribauer Maria, Marosi Christine, Birner Peter, Gatterbauer Brigitte, Fischer Ingeborg, Ströbel Thomas, Rössler Karl, Budka Herbert, Hainfellner Johannes A
Institute of Neurology, University of Vienna, AKH 4J, Währinger Gürtel 18-20, POB 48, Austria.
Eur J Cancer. 2002 Jul;38(10):1343-7. doi: 10.1016/s0959-8049(02)00065-5.
Assessment of tumour cell proliferation in glioblastoma (GB) has been a topic of considerable research interest over the past decade. However, the correlation of tumour proliferation and patient outcome has yielded controversial results. In this study, we examined immunohistochemically, using paraffin-embedded tissue, the expression of the proliferation-related markers DNA topoisomerase IIalpha (TIIalpha) and Ki-67 antigen in a cohort of 114 GB patients treated consecutively with surgery and radiochemotherapy, and correlated the expression with patient outcome. The TIIalpha labelling index (LI) ranged between 5.2 and 87.2% (median: 25.6%). Survival analysis disclosed an association between high TIIalpha expression levels and prolonged survival (P=0.040, log-rank test). TIIalpha expression correlates closely with Ki-67 labelling index (R=0.927, P<0.001), which itself is predictive of patient survival (P=0.044). However, in multivariate analysis, only the Karnofsky performance status remained predictive of patient survival. We conclude that high expression of TIIalpha and Ki-67 appears to be associated with a prolonged survival in our cohort of GB patients.
在过去十年中，胶质母细胞瘤（GB）肿瘤细胞增殖的评估一直是相当受研究关注的课题。然而，肿瘤增殖与患者预后的相关性产生了有争议的结果。在本研究中，我们使用石蜡包埋组织通过免疫组织化学方法检测了114例接受手术及放化疗的连续GB患者队列中增殖相关标志物DNA拓扑异构酶IIα（TIIα）和Ki-67抗原的表达，并将该表达与患者预后相关联。TIIα标记指数（LI）在5.2%至87.2%之间（中位数：25.6%）。生存分析揭示高TIIα表达水平与生存期延长之间存在关联（P = 0.040，对数秩检验）。TIIα表达与Ki-67标记指数密切相关（R = 0.927，P < 0.001），而Ki-67标记指数本身可预测患者生存（P = 0.044）。然而，在多变量分析中，只有卡氏功能状态仍然可预测患者生存。我们得出结论，在我们的GB患者队列中，TIIα和Ki-67的高表达似乎与生存期延长相关。
