长柄艾氏蟹（十足目，对虾科）的消化蛋白酶及其与蜕皮的关系。
Digestive proteinases of Artemesia longinaris (Decapoda, Penaeidae) and relationship with molting.
作者信息
Fernández Gimenez A V, García-Carreño F L, Navarrete del Toro M A, Fenucci J L
机构信息
Departamento Ciencias Marinas, Facultad Ciencias Exactas y Naturales, CONICET, Universidad Nacional de Mar del Plata, Funes 3350, B7602AYL, Mar del Plata, Argentina.
出版信息
Comp Biochem Physiol B Biochem Mol Biol. 2002 Jul;132(3):593-8. doi: 10.1016/s1096-4959(02)00080-5.
Digestive proteinase activities of Artemesia longinaris were assayed at different stages of the molting cycle. Total proteolytic activity in the hepatopancreas was highest during postmolt. Trypsin and chymotrypsin activities were highest during intermolt. Specific inhibitors and zymograms of A. longinaris hepatopancreas extracts showed four trypsins (14.79, 15.49, 16.60, 17.38 kDa, respectively) and three chymotrypsins (21.38, 22.91, 27.54 kDa, respectively). Our results suggest that proteolytic activity in the hepatopancreas of A. longinaris is influenced by the molting cycle. Types and activity of prawn digestive enzymes constitute background information to further study the digestive abilities of these organisms and will lead to understanding their nutritional needs and feeding ecology.
在蜕皮周期的不同阶段测定了长柄艾蒿的消化蛋白酶活性。肝胰腺中的总蛋白水解活性在蜕皮后阶段最高。胰蛋白酶和胰凝乳蛋白酶活性在蜕皮间期最高。长柄艾蒿肝胰腺提取物的特异性抑制剂和酶谱显示有四种胰蛋白酶（分别为14.79、15.49、16.60、17.38 kDa）和三种胰凝乳蛋白酶（分别为21.38、22.91、27.54 kDa）。我们的结果表明，长柄艾蒿肝胰腺中的蛋白水解活性受蜕皮周期影响。对虾消化酶的类型和活性构成了进一步研究这些生物消化能力的背景信息，并将有助于了解它们的营养需求和摄食生态。
Zotero 插件