Mahajan Vinit B, Wei Cui, McDonnell Peter J
Department of Microbiology and Molecular Genetics, University of California-Irvine, Irvine, CA 92697, USA.
Invest Ophthalmol Vis Sci. 2002 Jul;43(7):2143-51.
To identify changes in gene expression in human corneal fibroblasts after exposure to interleukin-1alpha.
RNA was isolated from cultured human corneal fibroblasts after treatment with interleukin-1alpha and subjected to DNA microarray analysis. Changes in gene expression were determined by comparison with untreated cells in three independent experiments after a Bayesian statistical analysis of variance.
Changes in gene expression were reproducibly observed in 165 genes representing previously identified and novel chemokines, matrix molecules, membrane receptors, angiogenic mediators, and transcription factors that correlated with pathophysiological responses to inflammation. Dramatic increases in gene expression were observed with exodus-1 (CCL20), MMP-12, and RhoA.
DNA microarray analysis of the corneal fibroblast response to interleukin-1alpha provides important insight into modeling changes in gene expression and suggests novel therapeutic targets for the control of corneal inflammation.
确定人角膜成纤维细胞在暴露于白细胞介素-1α后基因表达的变化。
用白细胞介素-1α处理培养的人角膜成纤维细胞后分离RNA，并进行DNA微阵列分析。在对三个独立实验中未处理的细胞进行贝叶斯方差统计分析后，通过与未处理细胞比较来确定基因表达的变化。
在165个基因中可重复观察到基因表达的变化，这些基因代表了先前已确定的和新发现的趋化因子、基质分子、膜受体、血管生成介质和转录因子，它们与炎症的病理生理反应相关。观察到exodus-1（CCL20）、MMP-12和RhoA的基因表达显著增加。
对角膜成纤维细胞对白细胞介素-1α反应的DNA微阵列分析为模拟基因表达变化提供了重要见解，并为控制角膜炎症提出了新的治疗靶点。
