悬浮培养水稻细胞中茉莉酸响应基因RRJ1和RRJ2的cDNA克隆与特性分析
Cloning and characterization of cDNAs for the jasmonic acid-responsive Genes RRJ1 and RRJ2 in suspension-cultured rice cells.
作者信息
Sugimori Miho, Kiribuchi Kyoko, Akimoto Chiharu, Yamaguchi Takeshi, Minami Eiichi, Shibuya Naoto, Sobajima Hiroyuki, Cho Eun-Min, Kobashi Nobuyuki, Nojiri Hideaki, Omori Toshio, Nishiyama Makoto, Yamane Hisakazu
机构信息
Biotechnology Research Center, The University of Tokyo, Japan.
出版信息
Biosci Biotechnol Biochem. 2002 May;66(5):1140-2. doi: 10.1271/bbb.66.1140.
Two cDNA clones for jasmonic acid (JA)-responsive genes, RRJ1 and RRJ2, were isolated by differential screening from suspension-cultured rice cells treated with JA for 2 h. The putative RRJ1 protein is completely identical to that of a putative rice cystathionine gamma-lyase, while the putative RRJ2 protein is highly similar in sequence to a rice pyruvate decarboxylase, PDC1.
通过差异筛选，从用茉莉酸（JA）处理2小时的悬浮培养水稻细胞中分离出两个茉莉酸响应基因RRJ1和RRJ2的cDNA克隆。推测的RRJ1蛋白与推测的水稻胱硫醚γ-裂解酶完全相同，而推测的RRJ2蛋白在序列上与水稻丙酮酸脱羧酶PDC1高度相似。
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