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水稻12-氧代植物二烯酸还原酶1基因OsOPR1启动子中茉莉酸响应区域的鉴定。

Identification of a jasmonic acid-responsive region in the promoter of the rice 12-oxophytodienoic acid reductase 1 gene OsOPR1.

作者信息

Sobajima Hiroyuki, Tani Tomoyuki, Chujo Tetsuya, Okada Kazunori, Suzuki Kazuya, Mori Satoshi, Minami Eiichi, Nishiyama Makoto, Nojiri Hideaki, Yamane Hisakazu

机构信息

Biotechnology Research Center, The University of Tokyo, Tokyo, Japan.

出版信息

Biosci Biotechnol Biochem. 2007 Dec;71(12):3110-5. doi: 10.1271/bbb.70532. Epub 2007 Dec 7.

DOI:10.1271/bbb.70532
PMID:18071256
Abstract

The rice 12-oxophytodienoic acid reductase 1 gene (OsOPR1), isolated as a jasmonic acid (JA)-responsive gene, has been suggested to be involved in defense responses in rice. We identified a 19-base pair region that is essential to the JA-responsiveness of OsOPR1 by deletion and mutation analysis of the promoter by dual luciferase assay. This region contains possible recognition sites for basic leucine zipper transcription factors.

摘要

水稻12-氧代植物二烯酸还原酶1基因(OsOPR1)作为茉莉酸(JA)响应基因被分离出来,有人认为它参与水稻的防御反应。通过双荧光素酶测定法对启动子进行缺失和突变分析,我们鉴定出一个对OsOPR1的JA响应至关重要的19个碱基对区域。该区域包含碱性亮氨酸拉链转录因子的可能识别位点。

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