Yoshida Tadashi, Hachimura Satoshi, Ishimori Mina, Kinugasa Fumitaka, Ise Wataru, Totsuka Mamoru, Ametani Akio, Kaminogawa Shuichi
Department of Applied Biological Chemistry, University of Tokyo, Japan.
Biosci Biotechnol Biochem. 2002 May;66(5):963-9. doi: 10.1271/bbb.66.963.
The Th1 and Th2 preference induced by cells from the Peyer's patch (PP) and spleen (SPL) with various doses of an antigen was examined. The same splenic T cell receptor-transgenic CD4+ T cells were first incubated with PP or SPL cells in the presence of various doses of an antigen, and the cytokine response was observed after secondary stimulation. A Th2-type pattern was only obtained for primary stimulation at 10 microM of the antigen with PP cells, whereas a Th1 pattern was induced at both higher and lower concentrations. SPL cells in the presence of 0.1 to 1 microM of the antigen induced the secretion of Th2-type cytokines. Ten and 100 microM of the antigen plus SPL cells did not induce the release of a large quantity of cytokines. PP cells induced a different cytokine pattern at the antigen concentration that induced a similar level of T cell proliferation with SPL cells. Our findings suggest that the antigen-dose dependent development of Th1/Th2 cells is differentially modulated by the antigen-presentation function of cells in PP and SPL.
研究了不同剂量抗原作用下，派尔集合淋巴结（PP）和脾脏（SPL）细胞诱导的Th1和Th2偏好性。首先将相同的脾脏T细胞受体转基因CD4 + T细胞与PP或SPL细胞在不同剂量抗原存在的情况下共同孵育，二次刺激后观察细胞因子反应。仅在10微摩尔抗原存在下用PP细胞进行初次刺激时获得Th2型模式，而在较高和较低浓度下均诱导出Th1模式。在0.1至1微摩尔抗原存在下的SPL细胞诱导Th2型细胞因子的分泌。10和100微摩尔抗原加SPL细胞未诱导大量细胞因子的释放。PP细胞在与SPL细胞诱导相似水平T细胞增殖的抗原浓度下诱导出不同的细胞因子模式。我们的研究结果表明，Th1/Th2细胞的抗原剂量依赖性发育受到PP和SPL中细胞抗原呈递功能的差异调节。
