Aigaki T, Ohsako T, Toba G, Seong K, Matsuo T
Department of Biological Sciences, Tokyo Metropolitan University, Hachioji-shi, Japan.
J Neurogenet. 2001;15(3-4):169-78. doi: 10.3109/01677060109167374.
In Drosophila melanogaster, gain-of-function mutagenesis utilizing the GAL4-UAS system has been established, allowing identification of genes that may not be easily detectable by loss-of-function screening approaches. The conditional features of misexpression systems are especially useful for studying late-stage biological processes, such as those involving adult behavior or lifespan. The gene search system, incorporating a bidirectional misexpression vector, was used to screen for genes critical for longevity determination. We have identified several genes whose misexpression in adulthood extends the fly's lifespan. Phenotypic characterization of fly lines carrying a mis-expression vector, in conjunction with obtaining information about the genomic insertion sites, creates valuable resources for the systematic functional genomics in Drosophila.
在黑腹果蝇中，利用GAL4-UAS系统的功能获得性诱变已被确立，这使得鉴定那些通过功能丧失筛选方法可能不易检测到的基因成为可能。错误表达系统的条件性特征对于研究后期生物过程特别有用，例如那些涉及成虫行为或寿命的过程。包含双向错误表达载体的基因搜索系统被用于筛选对寿命决定至关重要的基因。我们已经鉴定出几个基因，其在成年期的错误表达会延长果蝇的寿命。携带错误表达载体的果蝇品系的表型特征，结合获得有关基因组插入位点的信息，为果蝇系统功能基因组学创造了宝贵资源。
