Kim Ki Hong, Kim Chun Soo
Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, Pusan, Korea.
Dis Aquat Organ. 2002 Apr 24;49(1):45-9. doi: 10.3354/dao049045.
The effect of cimetidine on the praziquantel concentration in the blood of the rockfish Sebastes schlegeli and the consequent effect on the treatment efficacy against Microcotyle sebastis were investigated. Fish were divided into 7 groups and orally administered praziquantel alone (200 and 100 mg kg(-1) body weight [BW]) or in combination with cimetidine (in doses of 200, 100 or 50 mg kg(-1) BW cimetidine with a praziquantel dose of 100 mg kg(-1) BW). The fish in the sixth group were coadministered 50 mg praziquantel and 200 mg cimetidine kg(-1) BW. The fish in the control group were administered only saline. At 24 h post-treatment, the plasma was analyzed for praziquantel by reversed-phase high-performance liquid chromatography (RP-HPLC) using diazepam as the internal standard, and the gills were examined to confirm the effectiveness of each treatment. The praziquantel concentration in plasma of fish administered 100 mg praziquantel + 200 mg cimetidine kg(-1) BW was not significantly different from that of fish treated with 200 mg praziquantel kg(-1) BW and was significantly (p < 0.05) higher (about 2 times) than that of fish administered 100 mg praziquantel kg(-1) BW. The group of fish administered 50 mg praziquantel + 200 mg cimetidine kg(-1) BW showed a similar plasma praziquantel concentration to that in the fish treated with 100 mg praziquantel kg(-1) BW. The treatment efficacies of the groups of fish coadministered 100 mg praziquantel kg(-1) BW and various concentrations of cimetidine (200, 100 and 50 mg kg(-1) BW) were not significantly different from that of the group of fish administered 200 mg praziquantel kg(-1) BW, but were significantly higher than those of the groups of fish fed 100 mg praziquantel kg(-1) BW alone or coadministered 50 mg praziquantel + 200 mg cimetidine kg(-1) BW.
研究了西咪替丁对许氏平鲉血液中吡喹酮浓度的影响以及对治疗许氏平鲉微茎吸虫病疗效的后续影响。将鱼分为7组，分别单独口服吡喹酮（200和100 mg kg⁻¹体重[BW]）或与西咪替丁联合给药（西咪替丁剂量为200、100或50 mg kg⁻¹ BW，吡喹酮剂量为100 mg kg⁻¹ BW）。第六组鱼同时给予50 mg吡喹酮和200 mg西咪替丁kg⁻¹ BW。对照组鱼仅给予生理盐水。治疗后24小时，以地西泮为内标，采用反相高效液相色谱法（RP-HPLC）分析血浆中的吡喹酮，并检查鳃以确认各治疗的有效性。给予100 mg吡喹酮 + 200 mg西咪替丁kg⁻¹ BW的鱼血浆中吡喹酮浓度与给予200 mg吡喹酮kg⁻¹ BW的鱼无显著差异，且显著高于（p < 0.05）给予100 mg吡喹酮kg⁻¹ BW的鱼（约2倍）。给予50 mg吡喹酮 + 200 mg西咪替丁kg⁻¹ BW的鱼组血浆吡喹酮浓度与给予100 mg吡喹酮kg⁻¹ BW的鱼相似。联合给予100 mg吡喹酮kg⁻¹ BW和不同浓度西咪替丁（200、100和50 mg kg⁻¹ BW）的鱼组治疗效果与给予200 mg吡喹酮kg⁻¹ BW的鱼组无显著差异，但显著高于单独给予100 mg吡喹酮kg⁻¹ BW或联合给予50 mg吡喹酮 + 200 mg西咪替丁kg⁻¹ BW的鱼组。
