Glowinski J, Glowinski S
Department of Vascular Surgery and Transplantology, Medical University of Bialystok, Poland.
Eur J Vasc Endovasc Surg. 2002 Jun;23(6):550-5. doi: 10.1053/ejvs.2002.1656.
to evaluate the content of lipid peroxidation products (expressed by the concentration of thiobarbituric acid-reactive substances; TBARS), the content of myeloperoxidase (MPO), and the localisation of xanthine oxidase (XO) in varicose veins (vv), varicose veins with superficial thrombophlebitis and unchanged saphenous veins.
varicose saphenous veins, varicose veins with superficial thrombophlebitis and normal saphenous veins obtained during varicose vein surgery on 36 patients as well as healthy saphenous veins from cadaver organ donors (control). Homogenates were prepared in which TBARS concentration and MPO content were determined. Immunohistochemical staining to detect XO was also performed.
the highest concentration of TBARS occurred in vv with superficial thrombophlebitis, the lowest in donor vein. The highest content of MPO was observed in vv and slightly lower - in varicose veins with thrombophlebitis. A positive reaction for XO was seen in vv wall endothelium. Specimens of vv with thrombophlebitis revealed strong, intense staining in endothelium as well as in vasa vasorum.
varicose veins, especially those complicated with superficial thrombophlebitis revealed increased free radical generation. Its sources might be neutrophils, and in vv complicated with superficial thrombophlebitis-xanthine oxidase.
评估脂质过氧化产物的含量（以硫代巴比妥酸反应性物质的浓度表示；TBARS）、髓过氧化物酶（MPO）的含量以及黄嘌呤氧化酶（XO）在静脉曲张（vv）、伴有浅静脉血栓性静脉炎的静脉曲张和未改变的隐静脉中的定位。
从36例患者的静脉曲张手术中获取的大隐静脉曲张、伴有浅静脉血栓性静脉炎的静脉曲张和正常隐静脉，以及来自尸体器官供体的健康隐静脉（对照）。制备匀浆并测定其中TBARS浓度和MPO含量。还进行了免疫组织化学染色以检测XO。
TBARS浓度最高出现在伴有浅静脉血栓性静脉炎的静脉曲张中，最低出现在供体静脉中。MPO含量最高在静脉曲张中观察到，在伴有血栓性静脉炎的静脉曲张中略低。在静脉曲张壁内皮中可见XO阳性反应。伴有血栓性静脉炎的静脉曲张标本在内皮以及血管滋养管中显示出强烈、密集的染色。
静脉曲张，尤其是那些并发浅静脉血栓性静脉炎的静脉曲张，显示出自由基生成增加。其来源可能是中性粒细胞，而在并发浅静脉血栓性静脉炎的静脉曲张中是黄嘌呤氧化酶。
