Tanaka Junko, Miyake Takaaki, Shimizu Tadashi, Wakayama Toshio, Tsumori Michihiro, Koshimura Kunio, Murakami Yoshio, Kato Yuzuru
Department of Medicine, Shimane Medical University, Izumo, Japan.
Int J Hematol. 2002 Jun;75(5):489-92. doi: 10.1007/BF02982111.
We investigated the effects of low-dose granulocyte colony-stimulating factor (G-CSF) on the mobilization of stem cells in 6 healthy subjects. When G-CSF was administered by continuous subcutaneous infusion at a rate of 72 microg/day for 5 days, the numbers of white blood cells and granulocytes rapidly increased to maximal levels. CD34+ cells were mobilized to the peripheral blood in 3 days, and the maximal level was reached 4 or 5 days after the start of treatment. We attempted to determine whether the levels of mobilized stem cells that we could obtain using this method would be sufficient for peripheral blood stem cell transplantation. Two of the 6 subjects complained of mild bone pain 4 or 5 days after the start of treatment, but the pain did not affect their daily activities. Only 1 abnormal result (for serum alkaline phosphatase) was found in the laboratory data. The present preliminary results have provided us with a framework for a prospective study comparing low-dose continuous infusion with conventional mobilization procedures.
我们研究了低剂量粒细胞集落刺激因子（G-CSF）对6名健康受试者干细胞动员的影响。当以72微克/天的速率连续皮下输注G-CSF 5天时，白细胞和粒细胞数量迅速增加至最高水平。CD34+细胞在3天内被动员至外周血，治疗开始后4或5天达到最高水平。我们试图确定使用这种方法获得的动员干细胞水平是否足以进行外周血干细胞移植。6名受试者中有2名在治疗开始后4或5天抱怨有轻度骨痛，但疼痛未影响其日常活动。实验室数据中仅发现1项异常结果（血清碱性磷酸酶）。目前的初步结果为我们提供了一个前瞻性研究的框架，以比较低剂量连续输注与传统动员程序。
