Gomes S A O, Graciano G L, Nogueira N F S, De Souza W, Garcia E S, Azambuja P
Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, Fiocruz, Av. Brazil 4365, Manguinhos, CEP 21045-900, Rio de Janeiro, RJ, Brazil.
J Invertebr Pathol. 2002 Feb;79(2):86-92. doi: 10.1016/s0022-2011(02)00011-3.
Studies on the effects of gamma radiation on the infectivity of Trypanosoma rangeli (strain H14) for the vector Rhodnius prolixus revealed that (i) the LD(50) (lethal dose for 50% of bugs) for uninfected insects was 4147 rads; (ii) irradiated insects with a dose of 1200 rads subsequently infected with the flagellates exhibited a mortality of 45%, while uninfected irradiated insects showed a mortality of 5%, and infected nonirradiated insects exhibited 10% mortality; (iii) flagellates were present in the hemolymph of irradiated insects 7 days postinfection (p.i.), while in nonirradiated insects the parasites appeared in the hemocoel 18 days p.i.; (iv) T. rangeli infection decreased the number of hemocytes significantly and induced the formation of nodules in the hemolymph of both irradiated and nonirradiated insects; and (v) gamma irradiation affected the ultrastructural organization of the epithelial cells of the small intestine, principally the perimicrovillar membranes and microvilli. In this paper, we discuss the significance of the intestinal microenvironment of R. prolixus with regard to its interaction with T. rangeli.
关于伽马辐射对克氏锥虫（H14株）感染传播媒介长红猎蝽感染力影响的研究表明：（i）未感染昆虫的LD（50）（使50%的虫子致死的剂量）为4147拉德；（ii）接受1200拉德剂量照射后再感染鞭毛虫的昆虫死亡率为45%，而未感染的受照射昆虫死亡率为5%，感染但未受照射的昆虫死亡率为10%；（iii）感染后7天，受照射昆虫的血淋巴中出现鞭毛虫，而未受照射昆虫的寄生虫在感染后18天出现在血腔中；（iv）克氏锥虫感染显著减少了血细胞数量，并在受照射和未受照射昆虫的血淋巴中诱导形成结节；（v）伽马辐射影响小肠上皮细胞的超微结构组织，主要是微绒毛周围膜和微绒毛。在本文中，我们讨论了长红猎蝽肠道微环境在其与克氏锥虫相互作用方面的意义。
