Scalera Fortunato, Fischer Tina, Schlembach Dietmar, Beinder Ernst
Department of Obstetrics and Gynaecology, University of Erlangen/Nuremberg, Universitaetsstrasse 21-23, 91054 Erlangen, Germany.
Clin Sci (Lond). 2002 Jul;103(1):53-7. doi: 10.1042/cs1030053.
This study was conducted to compare the effects of serum from healthy pregnant women and that from pregnant women with pre-eclampsia on oxidative stress in endothelial cells in culture. Human umbilical vein endothelial cells (HUVECs) were incubated with serum from 18 pre-eclamptic, 18 healthy pregnant and 18 healthy non-pregnant women for 24 h. The levels of reduced glutathione (GSH) and lipid peroxides (LPOs) were measured in endothelial cell lysates. Measurement of malondialdehyde in combination with 4-hydroxyalkenals has been used as an indicator of LPOs. Serum from healthy pregnant women decreased significantly the LPO content in HUVECs in comparison with serum from pre-eclamptic women and healthy non-pregnant women (30.7+/-6.6 compared with 39.3+/-10.9 and 41.0+/-12.7 pmol/mg of protein respectively; P<0.003 and P<0.01 respectively). No differences in GSH content between the three groups (18.3+/-2.1 nmol/mg of protein for healthy pregnant, 19.2+/-3.3 nmol/mg for pre-eclamptic and 18.3+/-2.0 nmol/mg for healthy non-pregnant women) were found. Thus serum from normal pregnant women contains a factor(s) that decreases oxidative stress in human endothelial cells. This mechanism might be altered in pre-eclampsia.
本研究旨在比较健康孕妇血清与子痫前期孕妇血清对培养的内皮细胞氧化应激的影响。将人脐静脉内皮细胞（HUVECs）分别与18例子痫前期孕妇、18例健康孕妇和18例健康非孕妇的血清孵育24小时。测定内皮细胞裂解物中还原型谷胱甘肽（GSH）和脂质过氧化物（LPOs）的水平。丙二醛与4-羟基烯醛的联合测定已被用作LPOs的指标。与子痫前期孕妇血清和健康非孕妇血清相比，健康孕妇血清显著降低了HUVECs中的LPO含量（分别为30.7±6.6与39.3±10.9和41.0±12.7 pmol/mg蛋白质；P分别<0.003和P<0.01）。三组之间的GSH含量没有差异（健康孕妇为18.3±2.1 nmol/mg蛋白质，子痫前期孕妇为19.2±3.3 nmol/mg，健康非孕妇为18.3±2.0 nmol/mg）。因此，正常孕妇血清中含有一种可降低人内皮细胞氧化应激的因子。子痫前期这种机制可能会改变。
