de Groot C J, Davidge S T, Friedman S A, McLaughlin M K, Roberts J M, Taylor R N
Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco School of Medicine 94143-0132.
Am J Obstet Gynecol. 1995 Mar;172(3):976-85. doi: 10.1016/0002-9378(95)90030-6.
We investigated differences in prostacyclin production by endothelial cells exposed to plasma from either preeclamptic women or normal pregnant women.
A case-control study of matched preeclamptic and normal pregnancies was used to compare prostacyclin synthesis by human umbilical vein endothelial cells incubated with pregnancy plasma for 24 hours. Prostacyclin concentrations in conditioned media were measured by radioimmunoassay of its stable metabolite (6-keto-prostaglandin F1 alpha). Human umbilical vein endothelial cell lysates were used to determine concentrations of the enzymes cyclooxygenase and prostacyclin synthase.
Prostacyclin production by human umbilical vein endothelial cells incubated with plasma from preeclamptic women was significantly greater than that by cells exposed to normal pregnancy plasma. Differences in prostacyclin production under the two experimental conditions could be explained neither by differences in enzyme mass nor activities of cyclooxygenase and prostacyclin synthase.
The stimulatory effect of preeclampsia plasma on prostacyclin biosynthesis in human umbilical vein endothelial cells appears to be manifested at a step(s) proximal to the activation of cyclooxygenase. Possible mechanisms are increased phospholipase A2, lipoprotein, or lipid peroxide activities in preeclampsia.
我们研究了暴露于子痫前期妇女或正常孕妇血浆中的内皮细胞生成前列环素的差异。
采用一项子痫前期与正常妊娠匹配的病例对照研究，比较人脐静脉内皮细胞与妊娠血浆孵育24小时后前列环素的合成情况。通过对其稳定代谢产物（6-酮-前列腺素F1α）进行放射免疫测定来检测条件培养基中前列环素的浓度。用人脐静脉内皮细胞裂解物来测定环氧化酶和前列环素合酶的浓度。
与人脐静脉内皮细胞与正常孕妇血浆孵育相比，与子痫前期妇女血浆孵育的细胞生成的前列环素显著更多。两种实验条件下前列环素生成的差异既不能用酶量的差异来解释，也不能用环氧化酶和前列环素合酶的活性差异来解释。
子痫前期血浆对人脐静脉内皮细胞前列环素生物合成的刺激作用似乎在环氧化酶激活的近端步骤表现出来。可能的机制是子痫前期中磷脂酶A2、脂蛋白或脂质过氧化物活性增加。
