Iseki Eizo, Takayama Noriko, Furukawa Yoshiko, Marui Wami, Nakai Toshiki, Miura Satoshi, Uéda Kenji, Kosaka Kenji
Department of Psychiatry, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
Neurosci Lett. 2002 Jul 5;326(3):211-5. doi: 10.1016/s0304-3940(02)00418-4.
Dementia with Lewy bodies brains were immunohistochemically investigated using anti-synphilin-1 antibodies. The alpha-synuclein-positive brainstem type and well-defined cortical type Lewy bodies (LB) were positive for synphilin-1, while ill-defined LB and LB-related neurites were negative, suggesting that synphilin-1 does not directly associate with alpha-synuclein. Synphilin-1-positive LB were double-positive for phosphorylated neurofilament. In addition, tau-positive neurofibrillary tangles (NFT) were positive for synphilin-1, while neuropil threads were negative. Immunoelectron microscopically, synphilin-1 was located on filamentous components in cortical type LB and on paired helical filaments in NFT. It is likely that synphilin-1 accumulates in the cell body according to the axonal transport blockage, and associates with abnormal cytoskeltons during the formation of LB or NFT, suggesting that synphilin-1 is non-specifically implicated in the formation of different neuronal cytoskeletal inclusions.
使用抗突触核蛋白-1抗体对路易体痴呆患者的大脑进行免疫组织化学研究。α-突触核蛋白阳性的脑干型和明确的皮质型路易小体（LB）对突触核蛋白-1呈阳性，而不明确的LB和与LB相关的神经突呈阴性，这表明突触核蛋白-1不直接与α-突触核蛋白相关联。突触核蛋白-1阳性的LB对磷酸化神经丝呈双阳性。此外，tau阳性的神经原纤维缠结（NFT）对突触核蛋白-1呈阳性，而神经毡丝呈阴性。免疫电子显微镜观察显示，突触核蛋白-1位于皮质型LB的丝状成分上以及NFT的双螺旋丝上。突触核蛋白-1可能根据轴突运输阻滞在细胞体内积累，并在LB或NFT形成过程中与异常细胞骨架相关联，这表明突触核蛋白-1非特异性地参与了不同神经元细胞骨架包涵体的形成。
