Katsuse Omi, Iseki Eizo, Marui Wami, Kosaka Kenji
Department of Psychiatry, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
J Neurol Sci. 2003 Jul 15;211(1-2):29-35. doi: 10.1016/s0022-510x(03)00037-6.
We investigated 10 dementia with Lewy bodies (DLB) brains showing various degrees of Lewy pathology using alpha-synuclein-immunohistochemistry, and morphologically divided cortical Lewy bodies (LB) into six developmental stages. Further, we demonstrated the ultrastructure of each stage of cortical LB using alpha-synuclein-immunoelectron microscopy. In the initial stage, alpha-synuclein accumulated in part of the neuronal cytoplasm without filamentous components, then formed LB and LB-related neurites composed of granulo-filamentous components. Finally, LB degraded to extracellular LB composed of loose filamentous components with involved astroglial processes. In addition, we immunohistochemically investigated the accumulation of axonal transported substances in cortical LB, and showed that APP, chromogranin-A, synphilin-1 and synaptophysin accumulated in cortical LB from stages 1, 2, 3 and 4, respectively. These findings suggest that chronic axonal transport blockage is implicated in the development of cortical LB in DLB brains.
我们使用α-突触核蛋白免疫组织化学方法研究了10例具有不同程度路易体病理改变的路易体痴呆（DLB）患者的大脑，并将皮质路易体（LB）在形态上分为六个发育阶段。此外，我们使用α-突触核蛋白免疫电子显微镜展示了皮质LB各阶段的超微结构。在初始阶段，α-突触核蛋白积聚在部分神经元细胞质中，无丝状成分，随后形成由颗粒状丝状成分组成的LB和LB相关神经突。最后，LB降解为细胞外LB，由松散的丝状成分和相关的星形胶质细胞突起组成。此外，我们通过免疫组织化学研究了轴突运输物质在皮质LB中的积聚情况，结果显示APP、嗜铬粒蛋白A、突触结合蛋白-1和突触素分别在第1、2、3和4阶段积聚在皮质LB中。这些发现表明，慢性轴突运输阻滞与DLB大脑中皮质LB的形成有关。
