Zhang Lu, Cai You-Ming, Zhuge Qiang, Zou Hui-Yu, Huang Min-Ren
Key Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University, Nanjing 210037, China.
Yi Chuan Xue Bao. 2002 Jun;29(6):549-54.
Xinjiang is a center of distribution and differentiation of genus Dianthus in China, and has a great deal of species resources. The sequences of ITS region (including ITS-1, 5.8S rDNA and ITS-2) of nuclear ribosomal DNA from 8 species of genus Dianthus wildly distributed in Xinjiang were determined by direct sequencing of PCR products. The result showed that the size of the ITS of Dianthus is from 617 to 621 bp, and the length variation is only 4 bp. There are very high homogeneous (97.6%-99.8%) sequences between species, and about 80% homogeneous sequences between genus Dianthus and outgroup. The sequences of ITS in genus Dianthus are relatively conservative. In general, there are more conversion than transition in the variation sites among genus Dianthus. The conversion rates are relatively high, and the ratios of conversion/transition are 1.0-3.0. On the basis of phylogenetic analysis of nucleotide sequences the species of Dianthus in China would be divided into three sections. There is a distant relationship between sect. Barbulatum Williams and sect. Dianthus and between sect. Barbulatum Williams and sect. Fimbriatum Williams, and there is a close relationship between sect. Dianthus and sect. Fimbriatum Williams. From the phylogenetic tree of ITS it was found that the origin of sect. Dianthusis is earlier than that of sect. Fimbriatum Williams and sect. Barbulatum Williams.
新疆是中国石竹属植物的分布和分化中心，拥有丰富的物种资源。通过对PCR产物直接测序，测定了新疆野生分布的8种石竹属植物核糖体DNA的ITS区（包括ITS-1、5.8S rDNA和ITS-2）序列。结果表明，石竹属植物ITS长度为617～621 bp，长度变异仅4 bp。种间序列同源性很高（97.6%～99.8%），石竹属与外类群间序列同源性约为80%。石竹属植物ITS序列相对保守。总体而言，石竹属植物变异位点上的颠换多于转换。颠换率较高，颠换/转换比为1.0～3.0。基于核苷酸序列的系统发育分析，中国石竹属植物可分为3个组。髯毛组与石竹组、髯毛组与繸裂组关系较远，石竹组与繸裂组关系较近。从ITS系统发育树发现，石竹组的起源早于繸裂组和髯毛组。
