Xu Pengfei, Han Yingpeng, Wu Junjiang, Lv Huiying, Qiu Lijuan, Chang Ruzhen, Jin Limei, Wang Jinsheng, Yu Anliang, Chen Chen, Nan Haiyang, Xu Xiuhong, Wang Ping, Zhang Dayong, Zhang Shuzhen, Li Wenbin, Chen Weiyuan
Soybean Research Institute of Northeast Agricultural University, Key Laboratory of Soybean Biology of Education Department, Harbin 150030, China.
J Genet Genomics. 2007 Feb;34(2):180-8. doi: 10.1016/S1673-8527(07)60019-8.
The internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear ribosomal DNA (nrDNA) was amplified via the PCR method in seventeen different isolates of Phytophthora sojae using the common primers of the ITS of fungi. Around 800 bp-1,000 bp fragments were obtained based on the DL2000 marker and the sequences of the PCR products were tested. Taking isolate USA as outgroup, the phylogenetic tree was constructed by means of maximum parsimony analysis, and the genetic evolution among isolates was analyzed. The results showed that there is a great difference between the base constitution of ITS1 and ITS2 among various isolates. The seventeen isolates are classified into three groups, and the isolates from the same region belong to the same group, which shows the variation in geography.
利用真菌ITS通用引物，通过PCR方法扩增了17个不同大豆疫霉菌株的核糖体DNA（nrDNA）的内部转录间隔区（ITS）区域（ITS1、ITS2和5.8S rDNA）。基于DL2000标记获得了约800 bp - 1000 bp的片段，并对PCR产物序列进行了检测。以美国分离株为外群，采用最大简约法构建系统发育树，分析各分离株间的遗传进化。结果表明，不同分离株ITS1和ITS2的碱基组成存在很大差异。17个分离株分为三组，同一地区的分离株属于同一组，显示出地理差异。
