Misteli T, Spector D L
Cold Spring Harbor Laboratory, NY 11724, USA.
Nat Biotechnol. 1997 Oct;15(10):961-4. doi: 10.1038/nbt1097-961.
The recent emergence of an autofluorescent protein, the green fluorescent protein (GFP), has opened the door for the convenient use of intact living cells and organisms as experimental systems in fields ranging from cell biology to biomedicine. We present an overview of some of the major applications of GFP, namely its use in protein tagging and in monitoring gene expression as well as its potential in a variety of biological screens.
一种自发荧光蛋白——绿色荧光蛋白（GFP）的近期出现，为在从细胞生物学到生物医学等诸多领域将完整的活细胞和生物体便捷地用作实验系统打开了大门。我们概述了GFP的一些主要应用，即其在蛋白质标记、监测基因表达方面的应用以及在各种生物学筛选中的潜力。
