Dal Ponte Donny B, Berman Scott S, Patula Vangie, Kleinert Leigh, Williams Stuart K
Biomedical Engineering Program, University of Arizona, 1501 North Campbell Avenue, Tucson, AZ 85724-5084, USA.
J Endovasc Ther. 2002 Jun;9(3):333-43. doi: 10.1177/152660280200900312.
To characterize the healing response associated with an experimental endovascular graft (EVG) by examining the lumen of the prosthesis and the native vessel wall responses after implantation in a canine model.
An endovascular graft (EVG) constructed of hookless self-expanding nitinol springs covered by an ultrathin Dacron fabric was placed in the abdominal aorta of 8 dogs following femoral artery catheterization. After 12 weeks, specimens were subjected to histological and immunocytochemical testing to quantitatively and qualitatively analyze the cellular makeup of the luminal and abluminal tissues.
Gross examination revealed a glistening, thrombus-free luminal surface on all prostheses, which was confirmed by scanning electron microscopy. The EVGs were well incorporated into the aortic wall, leaving a concentric, smooth flow surface. Immunocytochemistry verified the presence of von Willebrand factor-positive endothelial cells on the luminal surface and alpha-smooth muscle cell actin-positive smooth muscle and/or fibroblast cells in the subendothelial space. A sparse inflammatory response and a paucity of proliferating nuclear cell antigen-positive cells were noted within the neomedial lining adjacent to the graft material. In addition, a rich vaso vasorum-like plexus of microvessels was evident within the neomedial tissue. The native vessel wall upon which the EVG impinged was largely unaffected. CONCLUSIONS; These data demonstrate that placement of an experimental EVG in the canine abdominal aorta elicits a healing response that is distinct from interpositional vascular grafts. Results from this study have particular relevance to the proximal and distal anastomotic neck regions.
通过检查假体管腔以及在犬模型中植入后天然血管壁的反应，来描述与实验性血管内移植物（EVG）相关的愈合反应。
在8只犬经股动脉插管后，将由超薄涤纶织物覆盖的无钩自膨式镍钛诺弹簧构成的血管内移植物（EVG）置于腹主动脉中。12周后，对标本进行组织学和免疫细胞化学检测，以定量和定性分析管腔和管腔外组织的细胞组成。
大体检查显示所有假体的管腔表面有光泽且无血栓，扫描电子显微镜证实了这一点。EVG很好地融入主动脉壁，留下一个同心、光滑的血流表面。免疫细胞化学证实管腔表面存在血管性血友病因子阳性内皮细胞，在内皮下间隙存在α-平滑肌肌动蛋白阳性的平滑肌和/或成纤维细胞。在与移植物材料相邻的新内膜衬里内观察到稀疏的炎症反应和增殖细胞核抗原阳性细胞数量较少。此外,在新内膜组织内可见丰富的类似血管滋养管的微血管丛。EVG所接触的天然血管壁基本未受影响。结论：这些数据表明，在犬腹主动脉中放置实验性EVG会引发一种不同于间置血管移植物的愈合反应。本研究结果与近端和远端吻合口颈部区域特别相关。
