Clark C Graham, Diamond Louis S
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, Great Britain.
Clin Microbiol Rev. 2002 Jul;15(3):329-41. doi: 10.1128/CMR.15.3.329-341.2002.
Cultivation of luminal protistan parasites has a long history. In this review we discuss the methods and media that are most widely used for the establishment and maintenance of the following organisms in culture: Entamoeba histolytica, Giardia intestinalis, Trichomonas vaginalis, Dientamoeba fragilis, Blastocystis hominis, and Balantidium coli. While cultivation is of limited importance in the diagnostic laboratory, it is essential to most research laboratories, and it is toward the latter that this review is primarily aimed.
管腔原生动物寄生虫的培养历史悠久。在本综述中,我们讨论了最广泛用于在培养中建立和维持以下生物体的方法和培养基:溶组织内阿米巴、肠贾第虫、阴道毛滴虫、脆弱双核阿米巴、人芽囊原虫和结肠小袋纤毛虫。虽然培养在诊断实验室中的重要性有限,但对大多数研究实验室来说却是必不可少的,而本综述主要针对的就是后者。