Moura-Oliveira Vivian, Oliveira Fabrício M S, Moreno Olga L M, Ferreira Julia R, Szawka Raphael E, Campideli-Santana Ana C, Teles Jullia, Capettini Luciano S A, Martins Flaviano S, Gomes Maria A
Department of Parasitology, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil.
Translational Type 2 Immunity Laboratory, Microbiology, Immunology and Tropical Medicine Department, George Washington School of Medicine and Health Sciences, Washington, DC 20052, USA.
Microorganisms. 2025 Apr 5;13(4):828. doi: 10.3390/microorganisms13040828.
Amebiasis is a globally prevalent infection that can lead to fatal outcomes if not adequately treated. Conventional treatment with imidazoles often fails due to side effects and resistance, emphasizing the need for alternative therapies. The probiotic Nissle 1917 (EcN) has shown potential in combating intestinal pathogens. This study aimed to evaluate the amebicidal activity of EcN in vitro and its effect on the production of reactive oxygen species (ROS). Trophozoites of (2.5 × 10⁴ cells/mL) were cultured in 96-well plates and exposed to varying concentrations of EcN (10-10 cells/mL). Plates were incubated at 36 °C for 6, 12, and 18 h, after which trophozoite viability was assessed. Intracellular ROS production, including superoxide and hydrogen peroxide, was measured using fluorescent probes. The highest efficacy was observed after 18 h at a CFU concentration of 10 cells/mL. Increased ROS production at all probiotic concentrations suggested a role in EcN's amebicidal mechanism. Morphological changes in trophozoites, such as rounding, vacuolization, and size reduction, were noted after EcN exposure, indicating growth inhibition. These findings suggest EcN induces structural and morphological changes in , inhibiting its growth in vitro. The findings suggest the potential efficacy of EcN; however, definitive confirmation requires data from human clinical trials.
阿米巴病是一种全球流行的感染性疾病，如果治疗不充分可能导致致命后果。由于副作用和耐药性，咪唑类药物的传统治疗常常失败，这凸显了对替代疗法的需求。益生菌Nissle 1917（EcN）已显示出对抗肠道病原体的潜力。本研究旨在评估EcN在体外的杀阿米巴活性及其对活性氧（ROS）产生的影响。将（2.5×10⁴个细胞/毫升）的滋养体接种于96孔板中，并暴露于不同浓度的EcN（10⁶ - 10⁸个细胞/毫升）。将平板在36℃下孵育6、12和18小时，之后评估滋养体的活力。使用荧光探针测量包括超氧化物和过氧化氢在内的细胞内ROS产生情况。在CFU浓度为10⁸个细胞/毫升、孵育18小时后观察到最高疗效。在所有益生菌浓度下ROS产生增加表明其在EcN的杀阿米巴机制中发挥作用。EcN暴露后观察到滋养体的形态变化，如变圆、空泡化和尺寸减小，表明生长受到抑制。这些发现表明EcN在体外可诱导溶组织内阿米巴的结构和形态变化，抑制其生长。这些发现提示了EcN的潜在疗效；然而，最终确认需要来自人类临床试验的数据。
