Visvesvara Govinda S, Garcia Lynne S
Centers for Disease Control and Prevention, Atlanta, Georgia 30341, Santa Monica, California 90402, USA.
Clin Microbiol Rev. 2002 Jul;15(3):327-8. doi: 10.1128/CMR.15.3.327-328.2002.
The in vitro culture of protozoan parasites involves highly complex procedures, which are subject to many variables. These parasites have very complex life cycles and, depending on the life cycle stage, may require different culture parameters. However, in vitro cultivation is important for many reasons, some of which include: diagnosis, antigen and antibody production, assessment of parasite immune modulating capabilities, drug screening, improvements in chemotherapy, differentiation of clinical isolates, determination of strain differences, vaccine production, development of attenuated strains, and the continued supply of viable organisms for studying host-parasite interactions.
原生动物寄生虫的体外培养涉及高度复杂的程序，这些程序受到许多变量的影响。这些寄生虫具有非常复杂的生命周期，并且根据生命周期阶段的不同，可能需要不同的培养参数。然而，体外培养由于多种原因而很重要，其中一些原因包括：诊断、抗原和抗体的产生、寄生虫免疫调节能力的评估、药物筛选、化疗的改进、临床分离株的鉴别、菌株差异的测定、疫苗生产、减毒株的开发，以及为研究宿主-寄生虫相互作用持续提供活的生物体。
