Øster Bodil, Höllsberg Per
Department of Medical Microbiology and Immunology, University of Aarhus, DK-8000 Aarhus C, Denmark.
J Virol. 2002 Aug;76(15):7578-86. doi: 10.1128/jvi.76.15.7578-7586.2002.
Herpesvirus gene expression is divided into immediate-early (IE) or alpha genes, early (E) or beta genes, and late (L) or gamma genes on the basis of temporal expression and dependency on other gene products. By using real-time PCR, we have investigated the expression of 35 human herpesvirus 6B (HHV-6B) genes in T cells infected by strain PL-1. Kinetic analysis and dependency on de novo protein synthesis and viral DNA polymerase activity suggest that the HHV-6B genes segregate into six separate kinetic groups. The genes expressed early (groups I and II) and late (groups V and VI) corresponded well with IE and L genes, whereas the intermediate groups III and IV contained E and L genes. Although HHV-6B has characteristics similar to those of other roseoloviruses in its overall gene regulation, we detected three B-variant-specific IE genes. Moreover, genes that were independent of de novo protein synthesis clustered in an area of the viral genome that has the lowest identity to the HHV-6A variant. The organization of IE genes in an area of the genome that differs from that of HHV-6A underscores the distinct differences between HHV-6B and HHV-6A and may provide a basis for further molecular and immunological analyses to elucidate their different biological behaviors.
疱疹病毒基因表达根据时间表达以及对其他基因产物的依赖性分为即刻早期（IE）或α基因、早期（E）或β基因以及晚期（L）或γ基因。通过实时PCR，我们研究了PL-1株感染的T细胞中35个人疱疹病毒6B（HHV-6B）基因的表达。动力学分析以及对从头蛋白质合成和病毒DNA聚合酶活性的依赖性表明，HHV-6B基因可分为六个独立的动力学组。早期表达的基因（I组和II组）和晚期表达的基因（V组和VI组）与IE和L基因非常吻合，而中间的III组和IV组包含E和L基因。尽管HHV-6B在整体基因调控方面具有与其他玫瑰疹病毒相似的特征，但我们检测到了三个B变异体特异性的IE基因。此外，不依赖从头蛋白质合成的基因聚集在病毒基因组中与HHV-6A变异体同源性最低的区域。基因组中与HHV-6A不同区域的IE基因组织强调了HHV-6B和HHV-6A之间的明显差异，并可能为进一步的分子和免疫学分析提供基础，以阐明它们不同的生物学行为。
