Kilberg Michael S, Barbosa-Tessmann Ione P
Department of Biochemistry and Molecular Biology, University of Florida College of Medicine, Gainesville 32610, USA.
J Nutr. 2002 Jul;132(7):1801-4. doi: 10.1093/jn/132.7.1801.
The human genes for C/EBP homology protein (chop) and asparagine synthetase (AS) are model systems to investigate transcription induced by nutrient limitation and endoplasmic reticulum (ER) stress. The genomic cis-elements in the promoters of these two genes that mediate these responses have been identified and partially characterized. Multiple cis-elements are functional in each gene, but differences exist in the molecular mechanisms by which these genes respond to amino acid or glucose deprivation. Whereas chop expression is associated with cell stress and apoptosis, activation of the AS gene by ER stress indicates that asparagine may also be critical for cellular processes other than protein synthesis.
C/EBP 同源蛋白（CHOP）和天冬酰胺合成酶（AS）的人类基因是用于研究营养限制和内质网（ER）应激诱导转录的模型系统。这两个基因启动子中介导这些反应的基因组顺式元件已被鉴定并部分表征。多个顺式元件在每个基因中都起作用，但这些基因对氨基酸或葡萄糖剥夺的反应分子机制存在差异。CHOP 的表达与细胞应激和凋亡相关，而 ER 应激对 AS 基因的激活表明，天冬酰胺可能对蛋白质合成以外的细胞过程也至关重要。
