Miller M A, Gardner I A, Packham A, Mazet J K, Hanni K D, Jessup D, Estes J, Jameson R, Dodd E, Barr B C, Lowenstine L J, Gulland F M, Conrad P A
Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616, USA.
J Parasitol. 2002 Jun;88(3):594-9. doi: 10.1645/0022-3395(2002)088[0594:EOAIFA]2.0.CO;2.
An indirect fluorescent antibody test (IFAT) for detection of Toxoplasma gondii infection was validated using serum from 77 necropsied southern sea otters (Enhydra lutris nereis) whose T. gondii infection status was determined through immunohistochemistry and parasite isolation in cell culture. Twenty-eight otters (36%) were positive for T. gondii by immunohistochemistry or parasite isolation or both, whereas 49 (64%) were negative by both tests. At a cutoff of 1:320, combined values for IFAT sensitivity and specificity were maximized at 96.4 and 67.3%, respectively. The area under the receiver-operating characteristic curve for the IFAT was 0.84. A titer of 1:320 was used as cutoff when screening serum collected from live-sampled sea otters from California (n = 80), Washington (n = 21), and Alaska (n = 65) for T. gondii infection. Thirty-six percent (29 out of 80) of California sea otters (E. lutris nereis) and 38% (8 out of 21) of Washington sea otters (E. lutris kenyoni) were seropositive for T. gondii, compared with 0% (0 out of 65) of Alaskan sea otters (E. lutris kenyoni).
采用间接荧光抗体试验（IFAT）检测刚地弓形虫感染，该试验使用了77只经尸检的南海獭（Enhydra lutris nereis）的血清进行验证，这些海獭的刚地弓形虫感染状况通过免疫组织化学和细胞培养中的寄生虫分离来确定。通过免疫组织化学或寄生虫分离或两者均检测，28只海獭（36%）刚地弓形虫呈阳性，而49只（64%）两项检测均为阴性。在截断值为1:320时，IFAT的敏感性和特异性综合值分别最大化至96.4%和67.3%。IFAT的受试者工作特征曲线下面积为0.84。在对从加利福尼亚（n = 80）、华盛顿（n = 21）和阿拉斯加（n = 65）现场采样的海獭血清进行刚地弓形虫感染筛查时，使用1:320的滴度作为截断值。加利福尼亚海獭（Enhydra lutris nereis）中36%（80只中的29只）和华盛顿海獭（Enhydra lutris kenyoni）中38%（21只中的8只）刚地弓形虫血清学呈阳性，而阿拉斯加海獭（Enhydra lutris kenyoni）中该比例为0%（65只中的零只）。
