Xie Mei-Lin, Mao Cai-Ping, Gu Zhen-Lun, Chen Ke-Ji, Zhou Wen-Xuan, Guo Ci-Yi
Department of Pharmacology, Faculty of Pharmacy, Soochow University, Suzhou 215007, China.
Acta Pharmacol Sin. 2002 Jul;23(7):597-600.
To investigate the mechanism of xiaoyu tablet on reduction of smooth muscle cells (SMC) in atherosclerotic vessel wall.
The atherosclerotic model was performed in male New Zealand rabbits that were given high fat diet and abrasion of the abdominal aorta endothelial cells. The rabbits were then administered with xiaoyu tablet 0.16-0.32 g x kg(-1) x d(-1) for 16 weeks. Changes in morphology, endothelin (ET)-1, nitric oxide (NO), and apoptotic cells of atherosclerotic vessel wall were determined by the microscopy, radioimmunoassay, colorimetric method, the techniques of DNA in situ end labeling, and image pattern analysis, respectively.
After 16 weeks of xiaoyu tablet treatment, intimal thickness and SMC in atherosclerotic vessel wall were diminished, ET-1 was decreased by 8.2 %-42.6 %, NO was increased by 7.5 %-54.2 %, and labeled apoptotic nuclei were markedly decreased, the area and integral optical density of positive granule were (846+/-308) microm2 and 3425+/-1374 in atherosclerotic group and (225+/-60) microm2 and 1445+/-606 in xiaoyu tablet 0.32 g/kg group, respectively.
Xiaoyu tablet not only inhibited proliferation of SMC through reducing ET-1 in atherosclerotic vessel wall, but also induced apoptosis of SMC by increasing NO in vessel wall.
探讨消瘀片减少动脉粥样硬化血管壁平滑肌细胞（SMC）的作用机制。
采用雄性新西兰兔，给予高脂饮食并损伤腹主动脉内皮细胞建立动脉粥样硬化模型。然后给兔灌胃消瘀片0.16 - 0.32 g·kg⁻¹·d⁻¹，连续16周。分别通过显微镜检查、放射免疫分析法、比色法、DNA原位末端标记技术及图像分析技术检测动脉粥样硬化血管壁的形态学变化、内皮素（ET）-1、一氧化氮（NO）及凋亡细胞情况。
消瘀片治疗16周后，动脉粥样硬化血管壁的内膜厚度及SMC减少，ET-1降低8.2% - 42.6%，NO升高7.5% - 54.2%，标记的凋亡细胞核明显减少，动脉粥样硬化组阳性颗粒面积及积分光密度分别为（846±308）μm²和3425±1374，消瘀片0.32 g/kg组分别为（225±60）μm²和1445±606。
消瘀片不仅通过降低动脉粥样硬化血管壁ET-1抑制SMC增殖，还通过增加血管壁NO诱导SMC凋亡。
