Maeda Nobuo, Senden Tim J, di Meglio Jean-Marc
Department of Applied Mathematics, Research School of Physical Sciences and Engineering, The Australian National University, Canberra, Australia.
Biochim Biophys Acta. 2002 Aug 19;1564(1):165-72. doi: 10.1016/s0005-2736(02)00443-1.
The molecular details of adhesion mechanics in phospholipid bilayers have been studied using atomic force microscopy (AFM). Under tension fused bilayers of dipalmitoylphosphatidylcholine (DPPC) yield to give non-distance dependent and discrete force plateaux of 45.4, 81.6 and 113+/-3.5 pN. This behaviour may persist over distances as great as 400 nm and suggests the stable formation of a cylindrical tube which bridges the bilayers on the two surfaces. The stability of this connective structure may have implications for the formation of pili and hence for the initial stage of bacterial conjugation. Dimyristoylphosphatidylcholine (DMPC) bilayers also exhibit force plateaux but with a much less pronounced quantization. Bilayers composed of egg PC, sterylamine and cholesterol stressed in a similar way show complex behaviour which can in part be explained using the models demonstrated in the pure lipids.
利用原子力显微镜（AFM）对磷脂双层中黏附力学的分子细节进行了研究。在张力作用下，二棕榈酰磷脂酰胆碱（DPPC）的融合双层会产生45.4、81.6和113±3.5 pN的与距离无关的离散力平台。这种行为可能在高达400 nm的距离上持续存在，表明形成了一个圆柱形管，该管桥接了两个表面上的双层。这种连接结构的稳定性可能对菌毛的形成有影响，从而对细菌接合的初始阶段有影响。二肉豆蔻酰磷脂酰胆碱（DMPC）双层也表现出力平台，但量化程度要小得多。以类似方式受力的由鸡蛋PC、硬脂胺和胆固醇组成的双层表现出复杂的行为，部分行为可以用纯脂质中展示的模型来解释。
