McDonald Alexander J, Mascagni Franco
Department of Cell Biology and Neuroscience, University of South Carolina School of Medicine, Bldg 1, Columbia, SC 29208, USA.
Brain Res. 2002 Jul 12;943(2):237-44. doi: 10.1016/s0006-8993(02)02650-1.
There are discrete subpopulations of GABAergic interneurons in the basolateral amygdala (ABL) that contain particular neuropeptides or calcium-binding proteins (calbindin-D28k, parvalbumin (PV), or calretinin). The present study employed a dual-labeling immunofluorescence technique combined with confocal laser scanning microscopy to investigate the neurochemical characteristics of the interneuronal subpopulation containing somatostatin (SOM). The great majority of SOM+ neurons in the ABL exhibited GABA immunoreactivity (66-82% depending on the nucleus). These SOM+ neurons constituted 11-18% of the GABA+ population. There was also extensive colocalization of SOM with calbindin (CB) in all nuclei of the ABL, but no colocalization of SOM with parvalbumin, calretinin, or vasoactive intestinal polypeptide. In the basolateral nucleus more than 90% of SOM+ neurons also exhibited CB immunoreactivity, whereas in the lateral nucleus about two-thirds of SOM+ neurons contained significant levels of CB. These SOM/CB neurons constituted about one quarter of the CB+ population in the basolateral nucleus and about one third of the CB+ population in the lateral nucleus. These results, in conjunction with the findings of previous studies, indicate that there are at least three major subpopulations of GABAergic interneurons in the ABL: (i) SOM+ neurons (most of which also contain CB and/or neuropeptide Y); (ii) PV+ neurons (most of which also contain CB); and (iii) CR+ neurons (most of which also contain vasoactive intestinal polypeptide).
基底外侧杏仁核（ABL）中存在离散的γ-氨基丁酸能中间神经元亚群，这些亚群包含特定的神经肽或钙结合蛋白（钙结合蛋白-D28k、小白蛋白（PV）或钙视网膜蛋白）。本研究采用双标记免疫荧光技术结合共聚焦激光扫描显微镜，来研究含有生长抑素（SOM）的中间神经元亚群的神经化学特征。ABL中绝大多数SOM+神经元表现出γ-氨基丁酸免疫反应性（取决于核团，比例为66%-82%）。这些SOM+神经元占γ-氨基丁酸+群体的11%-18%。在ABL的所有核团中，SOM与钙结合蛋白（CB）也有广泛的共定位，但SOM与小白蛋白、钙视网膜蛋白或血管活性肠肽没有共定位。在基底外侧核中，超过90%的SOM+神经元也表现出CB免疫反应性，而在外侧核中，约三分之二的SOM+神经元含有大量的CB。这些SOM/CB神经元在基底外侧核中约占CB+群体的四分之一，在外侧核中约占CB+群体的三分之一。这些结果与先前研究的发现相结合，表明ABL中至少有三个主要的γ-氨基丁酸能中间神经元亚群：（i）SOM+神经元（其中大多数也含有CB和/或神经肽Y）；（ii）PV+神经元（其中大多数也含有CB）；（iii）CR+神经元（其中大多数也含有血管活性肠肽）。
