用于检测抗猴出血热病毒抗体的酶联免疫吸附测定

Enzyme-linked immunosorbent assay for detection of antibodies against simian hemorrhagic fever virus.

作者信息

Godeny Elmer K

机构信息

Department of Pathological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803, USA.

出版信息

Comp Med. 2002 Jun;52(3):229-32.

PMID:12102567

Abstract

Better assays are needed for the detection of simian hemorrhagic fever virus (SHFV), which induces persistent infection without overt signs of disease in most old world monkeys, but causes a fatal hemorrhagic fever in macaques. An enzyme-linked immunosorbent assay (ELISA) is described here that is useful in identifying primates previously exposed to SHFV. This assay involves testing serum samples against SHFV and cell antigens to obtain an ODvirus-to-ODcell ratio that eliminates potential high background values associated with primate serum. High correlation was found using this assay, compared with that found with the current "gold standard" indirect immunofluorescence assay (IFA). However, this ELISA is less time consuming, less subjective, and not as prone to human error than the SHFV-IFA.

摘要

需要更好的检测方法来检测猴出血热病毒（SHFV），该病毒在大多数旧世界猴中会引发持续感染且无明显疾病迹象，但在猕猴中会导致致命的出血热。本文描述了一种酶联免疫吸附测定（ELISA）方法，该方法可用于识别先前接触过SHFV的灵长类动物。该检测方法包括针对SHFV和细胞抗原检测血清样本，以获得病毒OD值与细胞OD值的比率，从而消除与灵长类动物血清相关的潜在高背景值。与当前的“金标准”间接免疫荧光测定（IFA）相比，使用该检测方法发现具有高度相关性。然而，与SHFV-IFA相比，这种ELISA耗时更少、主观性更低，且不易出现人为误差。