Ye X Y, Ng T B
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
J Pept Res. 2002 Aug;60(2):81-7. doi: 10.1034/j.1399-3011.2002.20962.x.
A peptide, possessing a molecular mass of 5 kDa and demonstrating remarkable sequence homology to the cowpea 10 kDa protein precursor and garden pea disease resistance response protein, was isolated from rice bean seeds. The peptide was adsorbed on CM-Sepharose and Affi-gel blue gel. It inhibited mycelial growth in the fungi Botrytis cinerea, Fusarium oxysporum, Rhizoctonia solani and Mycosphaerella arachidicola. It stimulated incorporation of methyl [3H] thymidine into mouse splenocytes, inhibited the activity of human immunodeficiency virus type 1 reverse transcriptase and suppressed translation by rabbit reticulocyte lysate.
从饭豆种子中分离出一种分子量为5 kDa的肽，它与豇豆10 kDa蛋白前体和豌豆抗病反应蛋白具有显著的序列同源性。该肽可吸附于CM-琼脂糖和亲和蓝凝胶上。它能抑制灰葡萄孢、尖孢镰刀菌、立枯丝核菌和落花生球腔菌的菌丝生长。它能刺激甲基[3H]胸腺嘧啶核苷掺入小鼠脾细胞，抑制人类免疫缺陷病毒1型逆转录酶的活性，并抑制兔网织红细胞裂解物的翻译。
