Wang H, Ye X Y, Ng T B
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
Biol Chem. 2001 Jun;382(6):947-51. doi: 10.1515/BC.2001.118.
A novel antifungal protein, designated chrysancorin, was isolated from seeds of Chrysanthemum coronarium var. spatiosum with a procedure involving ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue resin, ion exchange chromatography on SP-Sepharose and FPLC-gel filtration on Superdex 75. The N-terminus of chrysancorin displays sequence similarity to the genomic sequence of chromosome 1 from Arabidopsis thaliana BAC T19E23. Chrysancorin exhibits a molecular mass of 13.4 kDa in gel filtration and SDS-polyacrylamide gel electrophoresis. It stimulates the proliferation of mouse splenocytes and inhibits the activity of human immunodeficiency virus-1 reverse transcriptase. The protein possesses antifungal activity against Botrytis cinerea, Mycosphaerella arachidicola and Physalospora piricola, but not against Rhizoctonia solani, Fusarium oxysporum and Coprinus comatus. However, we could not detect antibacterial activity against a variety of bacteria.
一种名为菊花抗真菌蛋白（chrysancorin）的新型抗真菌蛋白，是从茼蒿种子中分离得到的。其分离过程包括在DEAE - 纤维素上进行离子交换色谱、在Affi - gel蓝树脂上进行亲和色谱、在SP - Sepharose上进行离子交换色谱以及在Superdex 75上进行快速蛋白质液相色谱（FPLC）凝胶过滤。菊花抗真菌蛋白的N端序列与拟南芥BAC T19E23染色体1的基因组序列具有相似性。在凝胶过滤和SDS - 聚丙烯酰胺凝胶电泳中，菊花抗真菌蛋白的分子量为13.4 kDa。它能刺激小鼠脾细胞增殖，并抑制人类免疫缺陷病毒1逆转录酶的活性。该蛋白对灰葡萄孢、花生球腔菌和梨轮纹病菌具有抗真菌活性，但对立枯丝核菌、尖孢镰刀菌和鸡腿菇没有活性。然而，我们未检测到其对多种细菌的抗菌活性。
