来自海绵Haliclona cratera的一种新型凝集素：分离、表征及生物活性

A novel lectin from the sponge Haliclona cratera: isolation, characterization and biological activity.

作者信息

Pajic Ivana, Kljajic Zoran, Dogovic Nikola, Sladic Dusan, Juranic Zorica, Gasic Miroslav J

机构信息

Faculty of Chemistry, University of Belgrade, Studentski trg 16, P.O. Box 158, YU-11001, Belgrade, Yugoslavia.

出版信息

Comp Biochem Physiol C Toxicol Pharmacol. 2002 Jun;132(2):213-21. doi: 10.1016/s1532-0456(02)00068-6.

DOI:10.1016/s1532-0456(02)00068-6

PMID:12106898

Abstract

A lectin from the Adriatic sponge Haliclona cratera was purified by ion-exchange and gel chromatography. The molecular mass of the lectin is approximately 29 kDa. Purified lectin is rich in hydrophobic and basic amino acids and has an isoelectric point at pH 8.6. H. cratera lectin is relatively heat- and pH-stable. It agglutinates native and trypsinized, papainized and neuraminidase-treated human A, B, O, AB and sheep erythrocytes, and the hemagglutinating activity is independent of Ca(2+), Mn(2+) and Mg(2+) ions; D-galactose and N-acetyl-D-galactosamine are found to be moderate inhibitors of the activity. H. cratera lectin displays cytotoxic effect on HeLa and FemX cells and weak mitogenic effect on human T-lymphocytes pretreated with phytohemagglutinin (PHA).

摘要

通过离子交换和凝胶色谱法从亚得里亚海海绵Haliclona cratera中纯化出一种凝集素。该凝集素的分子量约为29 kDa。纯化后的凝集素富含疏水和碱性氨基酸，其等电点为pH 8.6。Haliclona cratera凝集素相对耐热且耐pH值变化。它能凝集天然的以及经胰蛋白酶处理、木瓜蛋白酶处理和神经氨酸酶处理的人A、B、O、AB型红细胞和绵羊红细胞，并且其血凝活性不依赖于Ca(2+)、Mn(2+)和Mg(2+)离子；发现D-半乳糖和N-乙酰-D-半乳糖胺是该活性的中度抑制剂。Haliclona cratera凝集素对HeLa细胞和FemX细胞具有细胞毒性作用，对用植物血凝素（PHA）预处理的人T淋巴细胞具有微弱的促有丝分裂作用。