Jeong Ji Hoon, Park Tae Gwan
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejon 305-701, South Korea.
J Control Release. 2002 Jul 18;82(1):159-66. doi: 10.1016/s0168-3659(02)00131-1.
Poly(lactic-co-glycolic acid) (PLGA)-grafted poly(L-lysine) (PLL) (PLL-g-PLGA) was synthesized to demonstrate its micelle-forming property in an aqueous solution. The micelles were used as a gene delivery carrier. The hydrodynamic diameter of PLL-g-PLGA micelles in an aqueous solution was ca. 149 nm with a narrow size distribution. Critical micelle concentration (cmc) was 9.6 mg/l. The PLL-g-PLGA micelles could be used to produce compact nanoparticulate complexes with plasmid DNA, which could efficiently protect the complexed DNA from enzymatic degradation by DNase I. The micelle/DNA complexes had highly compacted structure sized between 200-300 nm with a positive surface charge value. The PLL-g-PLGA micelles exhibited much higher transfection efficiency with lower cytotoxicity than PLL. Here, we demonstrated that biodegradable and cationic PLL-g-PLGA micelles could be used as an effective DNA condensation carrier for gene delivery system.
合成了聚（乳酸 - 乙醇酸）（PLGA）接枝的聚（L - 赖氨酸）（PLL）（PLL - g - PLGA），以证明其在水溶液中的胶束形成特性。这些胶束用作基因传递载体。PLL - g - PLGA胶束在水溶液中的流体动力学直径约为149 nm，粒径分布较窄。临界胶束浓度（cmc）为9.6 mg/l。PLL - g - PLGA胶束可用于与质粒DNA形成紧密的纳米颗粒复合物，从而有效地保护复合DNA免受DNase I的酶促降解。胶束/DNA复合物具有高度紧密的结构，尺寸在200 - 300 nm之间，表面带正电荷。与PLL相比，PLL - g - PLGA胶束表现出更高的转染效率和更低的细胞毒性。在此，我们证明了可生物降解的阳离子PLL - g - PLGA胶束可作为基因传递系统中有效的DNA凝聚载体。
